Addgene

We narrowed to 1,298 results for: sds

Showing: 51 - 100 of 1298 results

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pRPR1_c7gRNA_RPR1t

Plasmid

#64385

Purpose

encodes c7 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c7 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c1gRNA_RPR1t

Plasmid

#64379

Purpose

encodes c1 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c1 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_c5gRNA_RPR1t

Plasmid

#64383

Purpose

encodes c5 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

c5 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only
Construct 9 - U6p_30nt_stgRNA

Plasmid

#81248

Purpose

Expresses 30nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 11 - U6p_70nt_stgRNA

Plasmid

#81250

Purpose

Expresses 70nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 10 - U6p_40nt_stgRNA

Plasmid

#81249

Purpose

Expresses 40nt stgRNA

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPR1_g2gRNA_RPR1t

Plasmid

#64388

Purpose

encodes g2 gRNA

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

g2 gRNA

Use

Tags

Expression

Yeast

Mutation

Promoter

pRPR1

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pRPR1_gRNA_handle_RPR1t

Plasmid

#49014

Purpose

gRNA expression empty vector (yeast)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

pRPR1

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

Plasmid

#82024

Purpose

Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

mKate2-2xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSDM177

Plasmid

#194476

Purpose

pHERD30T-crRNA(Lse)_V3

Depositor

Joseph Bondy-Denomy

Article

Guan et al Nat Microbiol. 2022 Oct 31. pii: 10.1038

Insert

crRNA(Lse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCYC1m_yeGFP

Plasmid

#64389

Purpose

encodes yeast enhanced GFP under control of minimal pCYC1 promoter

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

pCYC1

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBI-GFP

Plasmid

#16623

Depositor

Bert Vogelstein

Article

Cahill et al Nature. 1998 Mar 19. 392(6673):300-3.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Construct 28 -hUBCp_TetR_p2a_LacI_p2a_ZeoR

Plasmid

#81253

Purpose

Lentiviral construct for building stable cell lines expressing TetR and LacI via Zeocin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

TetR and LacI

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 31 - 1xTetO_H1p_20nt2_stgRNA_3xLacO_U6p_20nt3_stgRNA

Plasmid

#81254

Purpose

For expressing Dox and IPTG inducible stgRNAs

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

stgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
1x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64390

Purpose

encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PCLP-2/pCMV (100)

Plasmid

#24421

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pEF6V5:eGFP-CAAX-2A-mCherry

Plasmid

#26901

Depositor

Steven Leach

Article

Provost et al Genesis. 2007 Oct . 45(10):625-9.

Insert

eGFP-CAAX-PTVI-2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
Construct 13 - CMVp_U6p_27nt1_GFP(+3)_RFP(+2)

Plasmid

#81252

Purpose

Mutation based toggling reporter (MTBR)

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Mutation Based Toggling Reporter (MBTR)

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440-daf-16a

Plasmid

#31503

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRPR1(1xTetO)_gRNA_handle_RPR1t

Plasmid

#62966

Purpose

aTc inducible gRNA expression vector

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440-daf-16df

Plasmid

#31505

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16d/f specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pBM5218

Plasmid

#18796

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Ty5-HIS3AI

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
L4440-daf-16abf

Plasmid

#31507

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/b/f common (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pET3_HMV-His6

Plasmid

#164843

Purpose

Expression of FL human metavinculin

Depositor

Tina Izard

Article

Rangarajan et al PLoS One. 2010 May 19;5(5):e10679. doi:

Insert

VCL (VCL Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440-daf-16b

Plasmid

#31504

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16b specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
Construct 3 - U6p_20nt1_mod2_sgRNA (stgRNA)

Plasmid

#81246

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440-daf-16af

Plasmid

#31506

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/f common (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
Construct 1 - U6p_20nt1_WT_sgRNA

Plasmid

#81245

Purpose

For assaying self-targeting activity via Cas9

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

sgRNA

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
12x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64393

Purpose

encodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

pCYC1

Available since

May 6, 2015

Availability

Academic Institutions and Nonprofits only
6x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64392

Purpose

encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
SPECS library

Pooled Library

#127842

Purpose

For performing high-throughput cell state-specific synthetic promoter screening

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Species

Synthetic

Use

Lentiviral

Available since

Aug. 22, 2020

Availability

Academic Institutions and Nonprofits only
pRS426GFP-2×PH(PLCδ)

Plasmid

#36092

Depositor

Scott Emr

Article

Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

Insert

2xPH(PLCdelta) fusion

Use

Tags

GFP

Expression

Yeast

Mutation

contains aa11-140 of PLCδ (including the PH domai…

Promoter

PRC1

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
NODAL-matMYC

Plasmid

#115248

Purpose

For mammalian expression of the human NODAL open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-BALF5

Plasmid

#72630

Purpose

Mammalian expression vector for Epstein-Barr virus DNA polymerase

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BALF5

Use

Tags

Expression

Mammalian

Mutation

G249S

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

Plasmid

#81251

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS424GFP-2xPH(Osh2)

Plasmid

#36095

Depositor

Scott Emr

Article

Stefan et al Cell. 2011 Feb 4;144(3):389-401.

Insert

2xPH(Osh2) fusion

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

PRC1

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pTPGI_dCas9

Plasmid

#64279

Purpose

encodes yeast-optimized dCas9 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

Yeast-optimized dCas9

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

D10A & H840A relative to S. pyogenes Cas9

Promoter

pTPGI

Available since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TRE3G-NODAL

Plasmid

#115638

Purpose

For targeted integration and inducible expression of human NODAL using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG5-BBLF4

Plasmid

#72634

Purpose

Mammalian expression vector for Epstein-Barr virus DNA helicase

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BBLF4

Use

Tags

Expression

Mammalian

Mutation

P5L

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pSG5-BSLF1

Plasmid

#69834

Purpose

Mammalian expression vector for Epstein-Barr virus primase BSLF1

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BSLF1

Use

Tags

Expression

Mammalian

Mutation

S2F

Promoter

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
LHP1463 - INP52

Plasmid

#32432

Depositor

Linda Hicke

Article

Stamenova et al J Biol Chem. 2004 Apr 16;279(16):16017-2

Insert

INP52 (INP52 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Lac

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSS2 (hS100B)

Plasmid

#26774

Depositor

Gary Shaw

Article

Smith et al Biochemistry. 1996 Jul 9. 35(27):8805-14

Insert

S100B (S100B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 7 - CMVp-Cas9-3xNLS-HSVpA

Plasmid

#81247

Purpose

Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1-TRE3G-NODALvar

Plasmid

#115640

Purpose

For targeted integration and inducible expression of a human NODAL splice variant using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NODAL-matDYK

Plasmid

#115258

Purpose

For mammalian expression of the human NODAL open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
NODALvar-matDYK

Plasmid

#115260

Purpose

For mammalian expression of a human NODAL splice varaint open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-matNODALvar-HIS

Plasmid

#115265

Purpose

For bacterial recombinant protein production of the mature peptide of a human NODAL splice variant

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL variant mature peptide (NODAL Human)

Use

Tags

6His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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