We narrowed to 157 results for: AsCas12a

1. pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

   Plasmid

   #160610

   Purpose

   Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   P35s:AsCas12a:T35s
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT7-AsCas12a_crRNA-site3 (RTW549)

   Plasmid

   #160138

   Purpose

   T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SM470_EF1A-dEnAsCas12a-T2A-EGFP

   Plasmid

   #244222

   Purpose

   Mammalian expression of dEnAsCas12a with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dEnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SM471_EF1A-dEnAsCas12a-T2A-BlastR

   Plasmid

   #244223

   Purpose

   Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Insert

   dEnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNS49 (enAsCas12a-KRAB piggyBac)

   Plasmid

   #217335

   Purpose

   piggyBac expresion of enAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   enAsCas12a
   

   Use

   CRISPR

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E174R/S542R/K548R

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2 AtU6-26:AsCas12a_DR (GB1442)

   Plasmid

   #160574

   Purpose

   AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtU6-26:AsCas12a_DR
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFP

   Plasmid

   #244218

   Purpose

   Mammalian expression of dEnAsCas12a-VPR with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dEnAsCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

   Plasmid

   #244219

   Purpose

   Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

   Depositor

   Tim Reddy

   Insert

   dEnAsCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AsCas12a-6xNLS-E174R/S542R (pRG232)

   Plasmid

   #149723

   Purpose

   Lentiviral expression plasmid of AsCas12a-6xNLS-E174R/S542R with puromycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid

    #160140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(nucleoplasmin)-3xHA-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

    Plasmid

    #244217

    Purpose

    Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFP

    Plasmid

    #244212

    Purpose

    Mammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

    Plasmid

    #244213

    Purpose

    Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

    Plasmid

    #244216

    Purpose

    Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SM280_EF1A-dEnAsCas12a-4xSID-T2A-BlastR

    Plasmid

    #244215

    Purpose

    Mammalian expression of dEnAsCas12a-4xSID with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-4xSID
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SM279_EF1A-dEnAsCas12a-4xSID-T2A-EGFP

    Plasmid

    #244214

    Purpose

    Mammalian expression of dEnAsCas12a-4xSID with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-4xSID
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-G-AsCas12a crRNA-BsaI cassette (MSP3491)

    Plasmid

    #114067

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of AsCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for AsCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-puro-U6-AsCas12a-TLR-MCV1-gRNA

    Plasmid

    #117411

    Purpose

    AsCas12a-gRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    AsCas12a gRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_mpx_AsCas12a_h7SK(PacI)_U6(I-SceI)_PGK-puro

    Plasmid

    #189633

    Purpose

    Lentiviral expression of dual AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas12a gRNA cassette, hU6 Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid

    #114069

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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