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We narrowed to 17,875 results for: puromycin

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Showing: 21 - 40 of 17875 results

  1. pBOB-EF1-FastFUCCI-Puro

    Plasmid
    #86849
    Purpose
    3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection
    Depositor
    Kevin Brindle, Duncan Jodrell
    Publication
    Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164
    Inserts
    mKO2-hCDT1(30-120)
    mAG-hGEM(1-110)
    pac (puromycin N-acetyl-transferase)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1 and PGK
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86849-plasmid-map-sequence-id-237968

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  3. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51760-plasmid-map-sequence-id-392672

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  7. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Publication
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112233-plasmid-map-sequence-id-213540

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  11. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid
    #78311
    Purpose
    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.
    Depositor
    Timo Otonkoski
    Publication
    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78311-plasmid-map-sequence-id-146188

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  12. pEF1a-FB-dCas9-puro

    Plasmid
    #100547
    Purpose
    Expresses N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9 protein
    Depositor
    Jian Xu
    Publication
    Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    N-terminal Flag and biotin-acceptor-site (FB)-tagged dCas9
    puromycin
    Use
    CRISPR
    Tags
    FLAG, Biotin-acceptor-site (Avitag)
    Expression
    Mammalian
    Promoter
    Human EF1a
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100547-plasmid-map-sequence-id-313417

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  16. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Publication
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99278-plasmid-map-sequence-id-262659

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  17. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Publication
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  19. pMB1610_pRR-Puro

    Plasmid
    #65853
    Purpose
    Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9
    Depositor
    Marc Bühler
    Publication
    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split puromycin N-acetyltransferase coding sequence
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65853-plasmid-map-sequence-id-112015

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Showing: 21 - 40 of 17875 results