We narrowed to 18,992 results for: Met;
-
Plasmid#79839PurposeExpresses RpBphP1-mCherry-Intersectin1 DHPH domain under CMVd1DepositorInsertRpBphP1-mCherry-Intersectin1 DHPH domain
UseSynthetic BiologyExpressionMammalianPromoterCMVd1Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
E.coli RaPID plasmid
Plasmid#107251PurposeExpresses the E.coli RaPID labeling proteinDepositorInsertE.coli BirA*
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAG-Pan
Plasmid#99640PurposeEGFP-mCherry Met71GAG reporter for methionine misincorporationDepositorInsertmCherry
TagsEGFPExpressionMammalianMutationMet71 mutated to GAG (Glu)PromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAA-Pan
Plasmid#99639PurposeEGFP-mCherry Met71GAA reporter for methionine misincorporationDepositorInsertmCherry
TagsEGFPExpressionMammalianMutationMet71 mutated to GAA (Glu)PromoterCMVAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PKA
Plasmid#60936PurposeFluorescent FPX sensor for cAMPDepositorInsertPKA FPX Sensor
ExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-Lux
Plasmid#109383PurposeArabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coliDepositorInsertpARABAD-LUX operon
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLys-M1
Plasmid#109382PurposeArabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
ExpressionBacterialAvailable SinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMVS1111A:PhmtB-bgaB
Plasmid#205998PurposeConjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidaseDepositorInsertthermostable beta-galactosidase
PromoterPhmtBAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-H3
Plasmid#109381PurposeArabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coliDepositorInsertpARABAD-VioB-mCherry
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHBA02
Plasmid#83536PurposeConstitutive TEF1 promoter mediated expression of the UBiC and ARO4* genesDepositorInsertpTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
ExpressionYeastPromoterpTEF1Available SinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2/SVNeo-103
Plasmid#79841PurposeExpresses RpBphP1-mCherry-TetR under CMVd1 promoterDepositorInsertRpBphP1-mCherry-Intersectin1 DHPH domain
ExpressionMammalianPromoterCMVd1Available SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CaYin1
Plasmid#33067DepositorInsertCaYin1
Tags6 His tagExpressionBacterialPromoterARA promoterAvailable SinceJan. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptac_BmHps_BmPhi_BsMdh
Plasmid#129103PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicusDepositorInsertBsMDH BmHPS BmPHI
ExpressionBacterialAvailable SinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
PylT(CTAG)WT PylS mCherry-P2A-eGFP(CTAG)
Plasmid#174527PurposeDual-fluorescence reporter for quantifying PylT(CTAG)WT decoding efficiencyDepositorInsertPylS
TagsFlagExpressionMammalianAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pD864-LacZ backbone
Plasmid#109384PurposeEmpty plasmid of the pD864-LacZ construct. This is high copy (pUC), AmpRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
PHBA01
Plasmid#83535PurposeConstitutive TEF1 promoter mediated expression of the UBiC geneDepositorInsertpTEF1-UBiC-CYC1t
ExpressionYeastPromoterpTEF1Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-ANME1BS MCR-NG
Plasmid#192772PurposeExpresses NG-tagged ANME1BS MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCW-SPI1
Plasmid#162824PurposeDoxycycline-inducible overexpression of human SPI1DepositorAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-GFP
Plasmid#66844PurposeTet-inducible expression of GFP in mammalian cellsDepositorInsertGFP
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPHT-PIP2
Plasmid#60937PurposeFluorescent FPX sensor for PIP2DepositorInsertPIP2 dimerization dependent sensor RA+B monomers
Tagsdimerization dependent fluorescent protein monome…ExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RA-NES
Plasmid#61019PurposeddRFP A-NESDepositorInsertddRFP A
TagsHindIII site and NES sequence MNLVDLQKKLEELELDEQQExpressionMammalianPromoterCMVAvailable SinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174529PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertsPylS
PylT
TagsFlagExpressionMammalianPromoterU6Available SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKA-207I10
Plasmid#79845PurposeExpresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoterDepositorInsertNLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
ExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
M3D395A-M14-dCas9
Plasmid#134780PurposeExpression of fusion protein M3D395A-M14-dCas9 in Mammalian cellsDepositorInsertMETTL3/METTL14 methyltransferase domain and inactive dCas9
ExpressionMammalianMutationD395APromoterCMVAvailable SinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE30-mBFP
Plasmid#112682PurposeExpression of NADPH-dependent blue fluorescent proteinDepositorInsertHis-mBFP
TagsHis tagExpressionBacterialPromoterT5Available SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-SPIC
Plasmid#162825PurposeDoxycycline-inducible overexpression of human SPICDepositorAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-GFE3
Plasmid#79869PurposeExpresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.DepositorInsertGFP-GFE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianPromoterBeta-actinAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K840R
Plasmid#204418PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
TagsHA and YFPExpressionMammalianMutationLysine 840 to Arginine (K840R)Available SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-YFP-Raptor K1097R
Plasmid#204417PurposeAcetylation mutant for human raptorDepositorInsertRegulatory Associated Protein Of MTOR, Complex 1
TagsHA and YFPExpressionMammalianMutationLysine 1097 to Arginine (K1097R)Available SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
groSL-GFP
Plasmid#109389PurposeGFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpgroSL-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPPC005
Plasmid#171140PurposeFor integration of XylS-Pm-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T methodDepositorInsertXylS-Pm
UseCRISPRExpressionBacterialPromoterPmAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC
Plasmid#175063Purposeexpresses mVenus and mCherry pair in mammalian cellsDepositorInsertmVenus + mCherry
ExpressionMammalianMutationmVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…PromoterCMV PromoterAvailable SinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
ExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ppst93-ANME2bHR1 MCR-NG
Plasmid#192774PurposeExpresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ppst93-ANME1G37 MCR-NG
Plasmid#192773PurposeExpresses NG-tagged ANME1G37 MCR in Methanococcus maripaludisDepositorInsertMethyl coenzyme M reductase
UseSynthetic Biology; Heterologous expression in met…TagsFlag-Strep2Available SinceDec. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
PylT(UAG) PylS mCherry-P2A-eGFP(150TAG)
Plasmid#174526PurposeDual-fluorescence reporter for quantifying PylT(UAG) decoding efficiencyDepositorInsertPylS
TagsFlagExpressionMammalianPromoterEF-1αAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-104
Plasmid#79842PurposeExpresses NLS-PpsR2-VP16 under CMV promoterDepositorInsertNLS-PpsR2-VP16
TagsNuclear localization signal of nuclear cap-bindin…ExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only