We narrowed to 1,257 results for: LIPI
-
Plasmid#102955PurposeMammalian expression of human soluble CD1D cDNADepositorInsertHuman soluble CD1D cDNA (CD1D Human)
UseTagsBirAExpressionMammalianMutationLacks the transmembrane regionPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_LacZ_sgRNA
Plasmid#155108Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LacZDepositorInsertLacZ_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-3HA-GK_v1
Plasmid#134275PurposeLentivector encoding 3XHA-tagged GK (isoform 1)DepositorInsertGk (Gk Mouse)
UseLentiviralTags3X HAExpressionMammalianMutation524 amino acids, isoform 1PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-LRRK1 K746G
Plasmid#233586PurposeExpresses LRRK1 K746G in mammalian cellsDepositorInsertLRRK1 K746G (LRRK1 Human)
UseTagsmScarletExpressionMammalianMutationPromoterAvailable sinceJune 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-InPTapp-Kras
Plasmid#235999PurposeGenetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to plasma membrane.DepositorInsertInPTapp-Kras (PLEKHA1 Synthetic, Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-InPGrp-Kras
Plasmid#236001PurposeGenetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.DepositorInsertInPGrp-Kras (Cyth3 Synthetic, Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Endo-InPGrp
Plasmid#236003PurposeGenetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.DepositorInsertEndo-InPGrp (Cyth3 Synthetic, Rat)
UseTags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-SS-VN155(I152L)-IRE1-dLD
Plasmid#217752PurposeExpresses the VN fragment of BiFC-IRE1-dLDDepositorInsertSerine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
UseTagsVenus (1-154, I152L)ExpressionMammalianMutationdeleted amino acids 29-408PromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-WzxE-TEV-His10
Plasmid#226781PurposeExpression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coliDepositorInsertwzxE
UseTagsTEV cleavable 10xHistidine tagExpressionBacterialMutationvaline insertion in position 2 due to the cloning…PromoteraraBADAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
mKate2-DrCavin1a in pT3TS-Dest
Plasmid#194294PurposeIn vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAIDepositorInsertcavin1a (cavin1a Zebrafish)
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3E-HsNRF2 (NFE2L2)
Plasmid#194302PurposeMultisite gateway entry clone for expression of human NRF2 (NFE2L2) with fusion tag at the N-terminus. Parton lab clone KRSDepositorInsertNFE2L2 (NFE2L2 Human)
UseMultisite gateway entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 [315-445]
Plasmid#186474PurposeExpression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseTags6HISExpressionBacterialMutationMSP domain [315-445]PromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-His6-K2P4.1A-mCherry-StreptagII
Plasmid#158743PurposeExpresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.DepositorInsertK2P4.1-A (KCNK4 Human)
UseTagsHis6 and mCherry with Streptag IIExpressionYeastMutationResidues 1-264 with N-linked glycosylation sites …PromoterAOX1Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_Luciferase_sgRNA
Plasmid#155109Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting LuciferaseDepositorInsertLuciferase_sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_Luciferase_sgRNA
Plasmid#155094Purposelentiviral plasmid expressing Cas9 and gRNA targeting LuciferaseDepositorInsertLuciferase_sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBOB-N-3HA-GK_v2
Plasmid#134277PurposeLentivector encoding 3XHA-tagged GK (isoform 2)DepositorInsertGk (Gk Mouse)
UseLentiviralTags3X HAExpressionMammalianMutation553 amino acids, isoform 2PromoterCMVAvailable sinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2-N[80]A
Plasmid#96995PurposeExpress GFP-tagged mutated mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationAmino acid residue 80 was mutated (N[80]A). Mutat…Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryExpressionMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_AAVS1_sgRNA_2
Plasmid#155105Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS_128
Plasmid#160651PurposeExpresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e3 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione3 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_101
Plasmid#160541PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_107
Plasmid#160650PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_129
Plasmid#160652PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterpZ estradiol inducible promoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-Rab18-sfGFP(N) HDR template
Plasmid#129415PurposeHDR tempalte for tagging of endogenous human RAB18 N-terminus with sfGFPDepositorInsertRAB18 HDR template (RAB18 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_AAVS1_sgRNA_2
Plasmid#155085Purposelentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMT-MjtRNA-GFP(47TAG)
Plasmid#193251PurposeExpresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)DepositorInsertsGFP(47TAG)
MjtRNA
UseTagsExpressionBacterialMutationCodon for Tyr47 replaced with amber stop codon TA…Promoterhsp60 and metUAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAzami-Green-Galectin
Plasmid#102419PurposeExpresses mAzami-labeled galectin 3 (Gal3) as a marker for endosomal leakage or rupture.DepositorInsertLGALS3 (LGALS3 Human)
UseTagsmAzami-greenExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-PTEN(A4)-mCherry
Plasmid#184054PurposeLysosome-targeted constitutively active mutant of phosphatase and tensin homolog (PTEN); tagged with mCherry.DepositorInsertLyso-PTEN(A4)-mCherry (PTEN Human)
UseTagsLysosome-associated membrane protein 1 (LAMP1). a…ExpressionMammalianMutationSer 380/385 and Thr 382/383 in PTEN mutated to Al…PromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-C-Flag-GK5_v2
Plasmid#134282PurposeExpresses Flag-tagged GK5 (isoform 2) in mammalian cellsDepositorInsertGk5 (Gk5 Mouse)
UseTagsFlagExpressionMammalianMutationmouse Gk5 isoform 2; 534 amino acidsPromoterCMVAvailable sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorUseTags6xHis-TEVExpressionBacterial and InsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast 3xFlag-SPNS1(E164K)
Plasmid#198569PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11
Plasmid#161923PurposeTo generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.DepositorInsertsgRNA targeting HSD17B11 exon 1
UseCRISPRTagsExpressionMutationPromoterU6Available sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast ACSL4
Plasmid#173164PurposeRetroviral vector to express sgRNA resistant human ACSL4DepositorInsertAcyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
UseRetroviralTagsExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast 3XFLAG-CHP1
Plasmid#173166PurposeRetroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1DepositorInsertCalcineurin Like EF-Hand Protein 1 (CHP1 Human)
UseRetroviralTags3XFLAGExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX304_C12orf49-V5
Plasmid#155110Purposelentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49DepositorInsertC12orf49 (SPRING1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterCMV promoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2
Plasmid#134288PurposeExpresses GK5 (isoform 2) in bacteriaDepositorInsertGk5 (Gk5 Mouse)
UseTagsMBPExpressionBacterialMutationmouse Gk5 isoform 2; 534 amino acidsPromoterT7Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mClover3_AAVS1_sgRNA_2
Plasmid#155099Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast HA-GPAT4
Plasmid#173168PurposeRetroviral vector to express sgRNA resistant HA tagged human GPAT4DepositorInsertGlycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
UseRetroviralTagsHAExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-FKBP-CYB5Atail
Plasmid#108141PurposeFor recruitment of ERDepositorUseTagsExpressionMammalianMutationCYB5A(100-134)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LCV2_AAVS1_sgRNA_3
Plasmid#155089Purposelentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_AAVS1_sgRNA_2
Plasmid#155088Purposelentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOET3-6xHis-hSMSr-ΔSAMD
Plasmid#236225PurposeExpress N-terminal His-tagged human SMSr (SAMD8)-sterile alpha motif domain deletion mutant protein in insect cellsDepositorInsertSAMD8 (SAMD8 Human)
UseTagsHexa histidine tag (6xHis-tag) and enterokinase s…ExpressionInsectMutationsterile alpha motif domain at N-terminal (1-231 b…PromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast 3xFlag-SPNS1(R76A)
Plasmid#198570PurposeSPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vectorDepositorInsertSPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
UseRetroviralTags3xFlagExpressionMammalianMutationchanged arginine R76 to alanine (R76A)PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IRES-blast GFP-SPNS1(E164K)
Plasmid#198572PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
UseRetroviralTags3xFlag-GFPExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)PromoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE-Truncated-Kir3.2-StreptagII
Plasmid#177263PurposeExpresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cellsDepositorInsertG-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
UseTagsStreptagIIExpressionInsectMutationResidues 49-378Promoterpolyhedrin promoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast ACSL3
Plasmid#173165PurposeRetroviral vector to express sgRNA resistant human ACSL3DepositorInsertAcyl-CoA Synthetase Long Chain Family Member 3 (ACSL3 Human)
UseRetroviralTagsExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mClover3_PSMD1_sgRNA_1
Plasmid#155102Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)DepositorInsertPSMD1_sgRNA_1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mClover3_AAVS1_sgRNA_4
Plasmid#155101Purposelentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCKO_AAVS1_sgRNA_3
Plasmid#155086Purposelentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LCV2_mCherry_AAVS1_sgRNA_4
Plasmid#155106Purposelentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locusDepositorInsertAAVS1_sgRNA_4
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only