We narrowed to 12,331 results for: 112

1. pARNTL-donor

   Plasmid

   #112384

   Purpose

   CRISPR donor plasmid to tag human transcription factor ARNTL with GFP

   Depositor

   Kevin White

   Insert

   ARNTL homology arms flanking EGFP-IRES-Neo cassette (BMAL1 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAT4

   Plasmid

   #112585

   Purpose

   Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5

   Plasmid

   #112840

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Full length Grb2

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hRIPK1-Flag-S320A

   Plasmid

   #112491

   Purpose

   Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S320A

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LUC45 (L/Sec/mutP)

   Plasmid

   #112148

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)
   

   Expression

   Mammalian

   Mutation

   Cysteine 258 mutated to selenocysteine (U) and SE…

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pESR1.1.0-gDNA

   Plasmid

   #112433

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1

   Depositor

   Kevin White

   Insert

   ESR1 (ESR1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pANY6

   Plasmid

   #112198

   Purpose

   This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.

   Depositor

   Yingfeng An

   Article

   Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

   Type

   Empty backbone

   Tags

   ELP-tag

   Expression

   Bacterial

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTORKm43GWC

   Plasmid

   #112898

   Purpose

   Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

   Depositor

   Sebastian Schornack

   Article

   Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

   Type

   Empty backbone

   Use

   Oomycete expression

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pR2Q347y

   Plasmid

   #112680

   Purpose

   functional, fluorescent, glutamate receptor subunit

   Depositor

   Thomas Hughes

   Article

   Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

   Insert

   Glutamate receptor subunit (Gria2 Rat)
   

   Tags

   fluorescent protein

   Expression

   Mammalian

   Mutation

   tagged with fluorescent protein

   Promoter

   cmV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/Puro/FA/GFP-LL5BIP

    Plasmid

    #112829

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

    Insert

    LL5BIP (2410002F23Rik Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-mEGFP-PKCg-C1

    Plasmid

    #112266

    Purpose

    ITRACKgamma Donor Construct

    Depositor

    Ryohei Yasuda

    Article

    Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

    Insert

    protein kinase c gamma (Prkcg Mouse)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTFE3.1.0-gDNA

    Plasmid

    #112473

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TFE3

    Depositor

    Kevin White

    Insert

    TFE3 (TFE3 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

    Plasmid

    #112215

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
    

    Use

    CRISPR

    Tags

    mCherry

    Mutation

    D10A,H840A,N863A,C710S

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SLF091: Lenti-CaMKIIa-eTsChR-eGFP

    Plasmid

    #112281

    Purpose

    For lentiviral or direct expression of eTsChR in excitatory neurons

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    eTsChR
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmiR-report-hEZH2

    Plasmid

    #112543

    Purpose

    3'UTR reporter assay

    Depositor

    Shusheng Wang

    Article

    Zhou et al Mol Ther. 2014 Feb;22(2):378-389. doi: 1

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Luciferase

    Available Since

    July 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTORKm43GWY

    Plasmid

    #112901

    Purpose

    Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

    Depositor

    Sebastian Schornack

    Article

    Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

    Type

    Empty backbone

    Use

    Oomycete expression

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.CAG-FLEX.iGABASnFR.F102G

    Plasmid

    #112167

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Mutation

    F102G

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORFE0011

    Plasmid

    #112075

    Purpose

    pcUBI promoter module Pro+5U

    Depositor

    Christian Chevalier

    Insert

    pcUBI promoter
    

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. FFTTH--GFP-Amotl2

    Plasmid

    #112833

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Insert

    Amotl2 (Amotl2 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOP3SU

    Plasmid

    #112599

    Purpose

    Used for expression of protein in E. coli as N-terminal Sumo tag fusions

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    Sumo

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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