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Showing: 4021 - 4040 of 31265 results

  2. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

    Plasmid
    #176577
    Purpose
    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:
    Insert
    Dispersin B
    Tags
    Hexahistidine tag and mVenus
    Expression
    Bacterial
    Mutation
    E184Q W330Y
    Promoter
    T7
    Available Since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176577-plasmid-map-sequence-id-348269

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  4. Tol2-GgVCL/WT

    Plasmid
    #162787
    Purpose
    CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    beta actin
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162787-plasmid-map-sequence-id-327628

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  6. PaCasp3

    Plasmid
    #166052
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-3 but function similar to caspase-6
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-3
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166052-plasmid-map-sequence-id-328270

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  7. PaCasp7a

    Plasmid
    #166051
    Purpose
    a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Porites astreiodes caspase-7a
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166051-plasmid-map-sequence-id-328269

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  18. pJK278

    Plasmid
    #71134
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Expression
    Bacterial
    Mutation
    changes histidine-89 to asparagine; additional N-…
    Promoter
    T7
    Available Since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71134-plasmid-map-sequence-id-131033

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  19. lentiCRISPR - C16orf80 sgRNA 1

    Plasmid
    #70651
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. lentiCRISPR - C16orf80 sgRNA 2

    Plasmid
    #70650
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C16orf80 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against C16orf80
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 4021 - 4040 of 31265 results