We narrowed to 31,602 results for: REP

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EBFP2-P2A-EGFP

Plasmid

#184938

Purpose

Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

EBFP2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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Donor-PIGA

Plasmid

#153530

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 1,991-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDendra2-H3

Plasmid

#157814

Purpose

Fluorescent reporter for histone H3 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H3 (H3c1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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2xHP1bCD_W42A-IFP2.0C

Plasmid

#215751

Purpose

Expresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xHP1bCD_W42A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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MacroH2A1.2-c002

Plasmid

#173088

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N372, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_mup53_Ex4

Plasmid

#85534

Purpose

Inducible expression of guide RNA (mup53_Ex4) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu p53

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pJP-HBB-nLuc

Plasmid

#175431

Purpose

Expresses nLuc with 5' leader of human beta globin (HBB) in mammalian cells.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

nLUC plus 5' HBB leader

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-NIrD

Plasmid

#65010

Purpose

NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

Depositor

Wensheng Wei

Article

Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

Inserts

rtTA-MAVS(C)
2A-linked delta-TK

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

Tight-TRE

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
hsp70: mKate2-DN Kif5a

Plasmid

#105972

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Tags

mKate2

Expression

Bacterial

Mutation

truncation of aa416-1033

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2ΔKinase

Plasmid

#163404

Purpose

Expresses BMPR-2 lacking the kinase domain in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The kinase domain (202-500 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

Plasmid

#159283

Purpose

A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Inserts

Human INS sgRNA
Human INS guide RNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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PGK ARF6(Q67L)-CFP

Plasmid

#200428

Purpose

Mammalian expression of Q67L mutation in ARF6

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

ARF6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Mutation

Q67L

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTet-O-OVOL1-T2A-PuroR

Plasmid

#162347

Purpose

Lentiviral expression of OVOL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

OVOL1 (OVOL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y419F

Plasmid

#140316

Purpose

Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Tags

2X HA

Expression

Mammalian

Mutation

Y419F

Promoter

CMV

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3

Plasmid

#62957

Purpose

Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4 in three phases

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2Δ751-813aa

Plasmid

#163603

Purpose

Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

The LIMK-binding region (751-813 aa) is deleted

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ-mNeongreen

Plasmid

#182392

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-LZ-mNeongreen

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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