We narrowed to 4,430 results for: Erf

1. MOM603

   Plasmid

   #133242

   Purpose

   expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

   Depositor

   Shinsuke Niwa

   Article

   Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

   Insert

   kif1a (KIF1A Human)
   

   Use

   Tags

   StrepII tag and mScarlet

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. plenti-CAG-IRF4-FLAG-IRES-GFP

   Plasmid

   #107389

   Purpose

   Lentiviral expression of IRF4-FLAG

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-UbC

   Plasmid

   #62806

   Purpose

   To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

   Depositor

   Pantelis Tsoulfas, Jae Lee

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Type

   Empty backbone

   Use

   AAV; Eucaryotic

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL7 lenti-dcas9-3xflag-blast

   Plasmid

   #112133

   Purpose

   lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   dCas9(D10A, N863A)-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A mutants in Cas9

   Promoter

   EF1a

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-UBC- IRF4-3xHA-pGK-PUR

   Plasmid

   #107386

   Purpose

   Lentiviral expression of IRF4-3xHA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IRF4 (IRF4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH0001

   Plasmid

   #85969

   Purpose

   UCOE-SFFV-dCas9-BFP-KRAB

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BFP-KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   SFFV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-UCOE-EF1a-Zim3-dCas9-BFP

   Plasmid

   #188775

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-SFFV-Zim3-dCas9-BFP

   Plasmid

   #188776

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH0006

    Plasmid

    #135448

    Purpose

    Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    dCas9-BFP-KRAB
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pdCas9-GFP

    Plasmid

    #181906

    Purpose

    Fluorescently tagged dead Cas9

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUW-TetO-Irf7

    Plasmid

    #178445

    Purpose

    doxycycline-inducible expression of Mouse Irf7 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    Irf7 (Irf7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

    Plasmid

    #208046

    Purpose

    Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    RNF4 (RNF4 Human)
    

    Use

    Lentiviral

    Tags

    BirAopt

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2-hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #225999

    Purpose

    To establish stable dCas9-KRAB knock-in line

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    humanized dCas9-KRAB T2A Puro
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGB 35s:dCas:BRD:tNos (GB1172)

    Plasmid

    #75401

    Purpose

    Transcriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional Repressor

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    dCas9:BRD
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOmniBac zz TEV YBBR TRF1

    Plasmid

    #185445

    Purpose

    Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Tags

    YBBR and ZZ

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-dCas9-T2A-GFP

    Plasmid

    #193781

    Purpose

    Tricistronic expression of dCas9-EGFP-BSD

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    dead Cas9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with existing Cas9 promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB-CAGGS-dCas9

    Plasmid

    #110823

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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