We narrowed to 23,628 results for: crispr

1. pCR8/GW:U6

   Plasmid

   #85981

   Purpose

   Golden Gate Level 0 cassette encoding the U6 promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   T. pseudonana U6 promoter
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Promoter

   none

   Available Since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHelper_AcCAST

   Plasmid

   #127923

   Purpose

   Expresses AcCAST and a crRNA targeting PSP1

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMiniT:GFP-Gephyrin TKIT donor

   Plasmid

   #169433

   Purpose

   Donor DNA for TKIT knockin of GFP to Gephyrin in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LRG2.1-mOrange

   Plasmid

   #124772

   Purpose

   3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJW1285

   Plasmid

   #61252

   Purpose

   CRISPR/Cas9 plasmid with sgRNA(F+E) targeting pha-1; for co-conversion in C. elegans

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E) targeting pha-1
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Gephyrin (px330:2-guideCas9)

   Plasmid

   #169432

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSHS212

   Plasmid

   #87346

   Purpose

   Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

   Depositor

   Jennifer Doudna

   Article

   Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

   Insert

   Cas9
   

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   C80S/C574S

   Promoter

   T7

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX853

   Plasmid

   #62885

   Purpose

   N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-573)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIF4

    Plasmid

    #196069

    Purpose

    Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

    Depositor

    Kabin Xie

    Article

    Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Promoter

    MoRP27 promoter

    Available Since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. bRA77

    Plasmid

    #100953

    Purpose

    GAL1 driven Cas9. gRNA can be cloned into BplI sites.

    Depositor

    James Haber

    Article

    Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT7sgRNA

    Plasmid

    #111820

    Purpose

    Expresses sgRNA in T. brucei cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    sgRNA
    

    Use

    CRISPR

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC1817_pCR8-gSMN2-EX

    Plasmid

    #118651

    Purpose

    Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-EX
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpyB

    Plasmid

    #220186

    Purpose

    sgRNA expression vector - pBG35 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pBG35

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

15. pOC1 cloning template vector

    Plasmid

    #183420

    Purpose

    CreON knock-in vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOC2 cloning template vector

    Plasmid

    #183421

    Purpose

    CreOFF knock-in vector

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJAT21

    Plasmid

    #204309

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPn
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJAT25

    Plasmid

    #204310

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPc
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKEW397_AcrIIA15

    Plasmid

    #138285

    Purpose

    Expression of AcrIIA15 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA15
    

    Use

    CRISPR

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKEW394_AcrIIA14

    Plasmid

    #138283

    Purpose

    Expression of AcrIIA14 protein

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA14
    

    Use

    CRISPR

    Tags

    SFGFP

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge