We narrowed to 12,126 results for: LEA
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Plasmid#140751PurposeInducing plant cells to enter mitosisDepositorAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only
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pFUW-tetO-BCL6
Plasmid#139816Purposedoxycycline-inducible expression of Human BCL6 in mammalian cellsDepositorInsertBCL6 (BCL6 Human)
UseLentiviralExpressionMammalianMutationT190A, E241G, L314M- please see depositor commentsPromoterTRE-mCMVAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p37)
Plasmid#135939PurposeExpresses AUF1p37 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-(STChRger2-TS-EYFP)
Plasmid#129394PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.DepositorInsertsoma targeted ChRger2
UseAAV and Cre/LoxTagsKv2.1-TS-EYFPExpressionMammalianPromoterCAGAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo
Plasmid#112684PurposeAn AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinaseDepositorInsertiRFP713
UseAAVTags2A-Flpo and Nuclear localization signalPromoterCMV-IEAvailable SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx1
Plasmid#116853PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 )
TagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationAmino acids 169.329Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
TagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_59.F2
Plasmid#60209PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Synthetic, Bacteriophage P1, Human)
TagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 19-59 o…PromoterCMVAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFB-GTE-COI1
Plasmid#29516DepositorInsertCOI1 (COI1 Mustard Weed)
TagsGST and TEV cleavage siteExpressionInsectMutationNote that the first AA in TEV site is R, not EAvailable SinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
dopamine beta hydroxylase (dbh)_L (OZ525)
Plasmid#27200DepositorInsertZinc finger array targeting dbh (dbh Zebrafish)
UseZebrafish targetingAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIS1 Cyclin D2 short UTR
Plasmid#21644DepositorAvailable SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TEV-U1AF37MF77M
Plasmid#168267PurposeExpresses human dmU1A with the F37M/F77M double mutantDepositorInsertU1A RRM1 crystallization module (SNRPA Human)
Tags6xHis tag N-terminus, TEV protease cleavage siteExpressionBacterialMutationchanged F37M and F77MPromoterT7 promotorAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
KU70-eGFP
Plasmid#213517PurposeExpresses human KU70-eGFP in mammalian cellsDepositorAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAIP Nup62Fv2GFP
Plasmid#139684PurposeNup62 dimerization construct fused to 2 copies of GFP under an SFFV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianPromoterSFFVAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCU-EGFP
Plasmid#181866PurposeExpresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV and AdenoviralTagsEGFPExpressionMammalianPromoterCaMKIIaAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133639
Plasmid#152344PurposeMammalian expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;3xFlagExpressionMammalianMutationcodon optimizedPromoterCMVAvailable SinceJune 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1(+)-ExRai-AKAR(T/A)
Plasmid#118406PurposeNegative-control mutant for ExRai-AKAR biosensor.DepositorInsertExRai-AKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only