We narrowed to 15,789 results for: LEA

1. pDG04439

   Plasmid

   #216365

   Purpose

   xhNup133-Nb2t (+2Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup133-Nb2t (+2Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Membrane EGFP

   Plasmid

   #198056

   Purpose

   Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence

   Depositor

   Amro Hamdoun

   Insert

   LCK membrane EGFP (LCK Human, Synthetic, A. victoria)
   

   Use

   Sea urchin, mammal, zebrafish

   Tags

   EGFP

   Expression

   Mutation

   Tandem repeat of N-terminal LCK membrane targetin…

   Promoter

   sp6

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSC226

   Plasmid

   #216375

   Purpose

   xhNup358-Nb2t (+3Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xhNup358-Nb2t (+3Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nanog5P reporter

   Plasmid

   #16337

   Depositor

   Austin Cooney

   Article

   Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

   Insert

   Nanog promoter (Nanog Mouse)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Nanog promoter

   Available since

   Dec. 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Vinculin-N-21

   Plasmid

   #55160

   Purpose

   Localization: Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Article

   Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

6. ND4.2-Left TALE-G1397-N-wildtype

   Plasmid

   #179684

   Purpose

   Expression of base editor in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXCas9H840A

   Plasmid

   #60900

   Purpose

   Dual Expression Vector for Cas9 H840A nickase and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Ran-Q69L-mRFP1-polyA

   Plasmid

   #104560

   Purpose

   Express Ran Q69L mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   Ran (Ran Mouse)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Q69L

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8xGliBS-IVS2-mCherry-NLS-Odc1-polyA-Tol2

   Plasmid

   #84603

   Purpose

   Tol2-based hedgehog signal reporter plasmid for zebrafish transgenesis

   Depositor

   James Chen

   Article

   Mich et al PLoS One. 2014 Jul 28;9(7):e103661. doi:

   Insert

   mCherry
   

   Use

   Zebrafish transgenesis

   Tags

   ODC1 destabilizing element

   Expression

   Mutation

   Promoter

   8xGliBS-delta-crystallin minimal promoter

   Available since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPM1003

    Plasmid

    #167550

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Ogawa serotype
    

    Use

    Cosmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-SisTnpB1–ωRNA

    Plasmid

    #228365

    Purpose

    Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

    Depositor

    Tao Liu

    Article

    Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

    Insert

    SisTnpB1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. ND4.2-Right DdCBE-G1397-C-wildtype

    Plasmid

    #179687

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3NES-CS(G)-tTA-CS(G)-3NES

    Plasmid

    #137836

    Purpose

    Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    3NES-CD(G)-tTA-CS(G)-3NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GRAB_r5-HTmut

    Plasmid

    #208720

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HTmut in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HTmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

    Plasmid

    #52549

    Purpose

    transgenic construct for expression of GFP and Flpo

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    Flpo recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUbi-MAD7

    Plasmid

    #170133

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin

    Plasmid

    #134857

    Purpose

    Expresses EGFP-tagged synaptic vesicle marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Ras-related protein 3C
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac_human-IL-18

    Plasmid

    #214338

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1

    Plasmid

    #169449

    Purpose

    expression of TRF1-mcherrry and sgRNA targeting telomeres

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    mCherry-TRF1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLS.400

    Plasmid

    #187201

    Purpose

    Expresses retron Eco4 RT

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    Retron Eco4 Reverse Transcriptase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mphR

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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