We narrowed to 30,517 results for: Ide

1. AAV2_CAG_oROS-G

   Plasmid

   #216115

   Purpose

   Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX307-VHL(Y98H)-3F-BirA

   Plasmid

   #220148

   Purpose

   Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   changed tyrosine 98 to histidine; codon optimized

   Promoter

   EF-1a

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. H52-pUCM-hNGN2-inv

   Plasmid

   #110492

   Purpose

   Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

   Depositor

   Michael Ward

   Inserts

   Neurogenin 2 (NEUROG2 Human)
   mcherry
   rtTA3G
   

   Use

   CRISPR, Cre/Lox, and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG, EF-1 alpha, and TRE3G

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY30

   Plasmid

   #84745

   Purpose

   Expresses huAsCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. SWEET11:SWEET11-2A-GFP-GUS

   Plasmid

   #172484

   Purpose

   Translational fusion of SWEET11 with a P2A self cleaving peptide sequence

   Depositor

   Wolf Frommer

   Article

   Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

   Insert

   SWEET11 (AT3G48740 Mustard Weed)
   

   Use

   Tags

   GFP, GUS

   Expression

   Plant

   Mutation

   Promoter

   SWEET11

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TRE3G-SOX17

   Plasmid

   #104541

   Purpose

   PiggyBac vector encoding doxycycline-inducible human SOX17

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   SRY-box 17 (SOX17 Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC3.1_CMV_oROS-G

   Plasmid

   #216111

   Purpose

   Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY31

   Plasmid

   #84746

   Purpose

   Expresses huLbCpf1-P2A-puro and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   puromycin resistance gene
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL0828 (pQCascade_crRNA-4)

   Plasmid

   #130637

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL1M-pMtRC-MtLAP1-Adh-R1

    Plasmid

    #170790

    Purpose

    A Root Tip-Specific Expressing Anthocyanin Marker for Direct Identification of Transgenic Tissues by the Naked Eye in Symbiotic Studies

    Depositor

    Jeremy Murray

    Article

    Ruan et al Plants (Basel). 2021 Mar 23;10(3). pii:

    Insert

    MtLAP1 (LOC11412013 Medicago truncatula)
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Medtr4g059670(MtRC)

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. QUAS-jGCaMP7s-T2A-tdTomato

    Plasmid

    #200010

    Purpose

    QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    3xP3-ECFP-jGCaMP7s-td-Tomato
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX Myc-HA

    Plasmid

    #229499

    Purpose

    For lentiviral expression of Myc coding sequence with an HA tag.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL0762 (pQCascade_crRNA-nt)

    Plasmid

    #130636

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Rory Johnson, Roderic Guigo

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

    Plasmid

    #211687

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid

    #200910

    Purpose

    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    3XHA_den1FnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Glutamic Acid to Arginine at 1369 position

    Promoter

    CBH

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-Gata2

    Plasmid

    #60665

    Purpose

    PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Gata2 (Gata2 Rat)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTarget TMEM97-P2A-mCardinal

    Plasmid

    #157747

    Purpose

    Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.

    Depositor

    Andrew Kruse

    Article

    Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114

    Insert

    TMEM97 (TMEM97 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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