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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,508 results for: crispr

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Showing: 4121 - 4140 of 25508 results

  1. cBEST3

    Plasmid
    #234659
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Publication
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and kasO17tss
    Available since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234659-plasmid-map-sequence-id-492747

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  5. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87700-plasmid-map-sequence-id-174647

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  7. M3-M14-dCas9

    Plasmid
    #134779
    Purpose
    Expression of fusion protein M3-M14-dCas9 in Mammalian cells
    Depositor
    Shu-Bing Qian
    Publication
    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    METTL3/METTL14 methyltransferase domain and inactive dCas9 (METTL3, METTL14 Human, S. pyogenes)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134779-plasmid-map-sequence-id-267134

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  8. pSC101-Donor

    Plasmid
    #140630
    Purpose
    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.
    Depositor
    Sheng Yang
    Publication
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Available since
    July 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    140630-plasmid-map-sequence-id-306011

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  9. pCas9-VRER_2A_GFP

    Plasmid
    #75475
    Purpose
    Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control
    Depositor
    Marc Tessier-Lavigne
    Publication
    Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VRER
    Use
    CRISPR
    Tags
    2A_GFP
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, T1337R
    Promoter
    CAG
    Available since
    May 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75475-plasmid-map-sequence-id-142912

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  11. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  13. U6-BbsI-DR_CMV-minidCas13X.1-REPAIRv2-BGHpA_CMV-EGFP-BGHpA

    Plasmid
    #171383
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-REPAIRv2 driven by CMV promoter,EGFP driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Publication
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized minidCas13X.1-REPAIRv2
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171383-plasmid-map-sequence-id-337350

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  14. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187960
    Purpose
    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187960-plasmid-map-sequence-id-373524

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  15. pX330 Ctnnb1.1

    Plasmid
    #59911
    Purpose
    pX330 backbone expressing sgRNA targeting Ctnnb1 to edit mouse beta-Catenin. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting Ctnnb1 (Ctnnb1 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    59911-plasmid-map-sequence-id-123316

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  19. pC0041-RanCas13b crRNA backbone

    Plasmid
    #103852
    Purpose
    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6-RanCas13b DR-BbsI-BbsI-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103852-plasmid-map-sequence-id-199153

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Showing: 4121 - 4140 of 25508 results