We narrowed to 52,828 results for: Ide

1. pH6-TEV-spTorA-GFP(C)

   Plasmid

   #169041

   Purpose

   GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   H6-spTorA-GFP
   

   Use

   Tags

   6xHis, TEV cleavage site, and spTorA (signal pept…

   Expression

   Bacterial

   Mutation

   The mut3 GFP variant with 5 additional mutations …

   Promoter

   araBAD

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP TEM4 FL

   Plasmid

   #58893

   Purpose

   Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.

   Depositor

   Channing Der

   Article

   Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

   Insert

   TEM4 (ARHGEF17 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L722F

   Promoter

   CMV

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL8 lenti-D10Acas9-3xflag-blast

   Plasmid

   #112134

   Purpose

   lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   D10Acas9-3xflag-blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A mutant in Cas9

   Promoter

   EF1a

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_VIP1.0

   Plasmid

   #208682

   Purpose

   Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

   Plasmid

   #71216

   Purpose

   dCas9 fused to human DNMT3A catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A catalytic domain
   

   Use

   Lentiviral

   Tags

   dSpCas9-DNMT3A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. eSpCas9-plus

   Plasmid

   #126767

   Purpose

   Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   K848A, R1060A; amino acids 1005-1013 replaced wit…

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL4 sgRNA(MS2)-MS2-hA3a

   Plasmid

   #112130

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a (APOBEC3A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6,CBh

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

   Plasmid

   #71217

   Purpose

   dCas9 fused to human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B catalytic domain
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

    Plasmid

    #71218

    Purpose

    dCas9 fused to the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A(E752A)
    

    Use

    Lentiviral

    Tags

    SPdCas9-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDisplay-GRAB_NPYmut

    Plasmid

    #208678

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Rat SERCA2a (pMT2)

    Plasmid

    #75183

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, Gary Shull

    Article

    Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

    Insert

    ATP2A2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM33_Orf9b

    Plasmid

    #156485

    Purpose

    Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf9b
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-GRAB_NPYmut

    Plasmid

    #208679

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. VP16-ER beta (short)

    Plasmid

    #11353

    Depositor

    Donald McDonnell

    Article

    Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

    Insert

    ER beta short (ESR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

    Plasmid

    #176535

    Purpose

    Non-protein coding locus components from CRISPR-associated IscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-B-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207385

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP TEM4 R130D

    Plasmid

    #58895

    Purpose

    Expresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.

    Depositor

    Channing Der

    Article

    Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

    Insert

    TEM4 (ARHGEF17 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R130D, L722F

    Promoter

    CMV

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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