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We narrowed to 5,632 results for: chia

Showing: 1001 - 1050 of 5632 results

  2. THI ONLY pALD4 T-

    Plasmid
    #161497
    Purpose
    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pALD4, pPOR1, and pTEF2
    Available since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161497-plasmid-map-sequence-id-326273

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  6. pET22-HT/purC

    Plasmid
    #73623
    Purpose
    Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag
    Depositor
    James Williamson, T. J. Kappock
    Article
    Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.
    Insert
    phosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Contains multiple silent mutations (E179, G185, G…
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73623-plasmid-map-sequence-id-136194

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  7. pJK048

    Plasmid
    #75230
    Purpose
    Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    JoAnne Stubbe, T. J. Kappock
    Article
    Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…
    Promoter
    T7 and n/a
    Available since
    Availability
    Academic Institutions and Nonprofits only
    75230-plasmid-map-sequence-id-142240

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  8. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Article
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PA1
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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  10. pUC-GFP-MCC

    Plasmid
    #133307
    Purpose
    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation
    Depositor
    Chris Barnes
    Article
    Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1
    Insert
    microcin-V bacteriocin cassette (cvaC E. coli)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    N112D (please see depositors comments)
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    133307-plasmid-map-sequence-id-272413

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  12. pRSF-Ivy-his-hLYZ

    Plasmid
    #83920
    Purpose
    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.
    Depositor
    Karl Griswold
    Article
    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do
    Inserts
    Ivy
    lysozyme (LYZ Human)
    Use
    Tags
    Ivy protein has C-terminal hisx6 tag
    Expression
    Bacterial
    Mutation
    Ivy leader sequence deleted and replaced.
    Promoter
    T7 and t7
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    83920-plasmid-map-sequence-id-160356

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  16. pTUM4

    Plasmid
    #230905
    Purpose
    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli
    Depositor
    Arne Skerra
    Article
    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-
    Inserts
    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    230905-plasmid-map-sequence-id-460506

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  22. pGL403

    Plasmid
    #119953
    Purpose
    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102
    Inserts
    gpps
    limS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminal truncation of 56 amino acid residues (…
    Promoter
    Available since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    119953-plasmid-map-sequence-id-238006

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  23. pGL

    Plasmid
    #119952
    Purpose
    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102
    Inserts
    gpps
    limS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminal truncation of 56 amino acid residues (…
    Promoter
    Available since
    Feb. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119952-plasmid-map-sequence-id-235949

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  25. pMVP-SEC24C

    Plasmid
    #231559
    Purpose
    pMVP expression vector for human SEC24C (wild type, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    silent PAM mutations (G67 (GGG>GGA) and A115 (…
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231559-plasmid-map-sequence-id-459740

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  26. pMVP-SEC24C-G220C

    Plasmid
    #231560
    Purpose
    pMVP expression vector for human SEC24C (G220C mutation, closed, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (G220C) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    G220C, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231560-plasmid-map-sequence-id-460046

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  27. pMVP-SEC24C-S107F

    Plasmid
    #231561
    Purpose
    pMVP expression vector for human SEC24C (S107F mutation, closesd, resistant to sgSEC24C #1 and #2) (Blasticidin selection marker)
    Depositor
    Rizwan Haq
    Article
    Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e
    Insert
    SEC24C (S107F) (SEC24C Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    S107F, silent PAM mutations (G67 (GGG>GGA) and…
    Promoter
    CMV
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231561-plasmid-map-sequence-id-459737

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  32. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

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  34. pGLU_116

    Plasmid
    #225796
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225796-plasmid-map-sequence-id-447051

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  35. pGLU_80

    Plasmid
    #225781
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225781-plasmid-map-sequence-id-447019

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  36. pGLU_98

    Plasmid
    #225811
    Purpose
    RS421 library variant 14271
    Depositor
    Nili Ostrov
    Insert
    14271_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225811-plasmid-map-sequence-id-446973

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  37. pKP864

    Plasmid
    #167658
    Purpose
    Express colicinB-His6
    Depositor
    Kathleen Postle
    Insert
    cba-his6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167658-plasmid-map-sequence-id-337304

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  38. pKP1392

    Plasmid
    #167649
    Purpose
    Express ExbD T42C protein
    Depositor
    Kathleen Postle
    Insert
    exbD T42C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbD with T42C mutation
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167649-plasmid-map-sequence-id-337289

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  39. pJOE6087.3

    Plasmid
    #135082
    Purpose
    pBR322-based L-rhamnose-inducible vector.
    Depositor
    Josef Altenbuchner
    Insert
    P-rhaP-BAD-eGFP-His tag
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135082-plasmid-map-sequence-id-266686

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  40. pKP968

    Plasmid
    #167653
    Purpose
    Express colicinB-His6, D17C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 D17C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with D17C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167653-plasmid-map-sequence-id-339836

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  41. pKP996

    Plasmid
    #167654
    Purpose
    Express colicinB-His6, T18C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 T18C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with T18C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167654-plasmid-map-sequence-id-339834

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  42. pKP995

    Plasmid
    #167655
    Purpose
    Express colicinB-His6, M19C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 M19C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with M19C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167655-plasmid-map-sequence-id-339832

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  43. pKP997

    Plasmid
    #167656
    Purpose
    Express colicinB-His6, V20C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 V20C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with V20C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167656-plasmid-map-sequence-id-339829

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  44. pKP2264

    Plasmid
    #167660
    Purpose
    Express colicinB-His6, A121C, Y254C
    Depositor
    Kathleen Postle
    Insert
    cba-his6, A121C, Y254C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6, I121C, Y254C
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167660-plasmid-map-sequence-id-337305

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  45. pKP579

    Plasmid
    #159598
    Purpose
    Express Cba and Cbi protein
    Depositor
    Kathleen Postle
    Insert
    cba and cbi
    Use
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    159598-plasmid-map-sequence-id-313311

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  46. pKP1912

    Plasmid
    #167600
    Purpose
    Express ExbB delta(235-244),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 235-24…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167600-plasmid-map-sequence-id-339838

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  47. pKP2040

    Plasmid
    #167650
    Purpose
    Express His6-ExbB S80A, S82A, S84A
    Depositor
    Kathleen Postle
    Insert
    his6-exbB S80A, S82A, S84A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    ExbB S80A, S82A, S84A with His6 tag at N terminus
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167650-plasmid-map-sequence-id-337301

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  48. pKP1940

    Plasmid
    #167599
    Purpose
    Express ExbB delta(225-234),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 225-23…
    Promoter
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    167599-plasmid-map-sequence-id-333697

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  49. pKP1965

    Plasmid
    #167598
    Purpose
    Express ExbB delta(215-224),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 215-22…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167598-plasmid-map-sequence-id-332908

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  50. pKP1964

    Plasmid
    #167597
    Purpose
    Express ExbB delta(205-214),ExbD
    Depositor
    Kathleen Postle
    Insert
    ExbB, ExbD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    exbB gene with in-frame deletion of codons 205-21…
    Promoter
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    167597-plasmid-map-sequence-id-332907

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Showing: 1001 - 1050 of 5632 results