We narrowed to 14,002 results for: flag
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pMRX-IPU-GFP-NEK9W967A
Plasmid#168271PurposeExpresses LIR (LC3-interacting region) mutant NEK9DepositorInsertNEK9 (NEK9 Human)
UseTagsFLAGExpressionMammalianMutationchanged tryptophan 979 to alaninePromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1333-C
Plasmid#174557PurposeChloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vectorDepositorInsertDdCBE G1333-C
UseTagsFLAGExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_GATAD1
Plasmid#104036PurposeDonor vector for 3' FLAG tag of human GATAD1DepositorInsertGATAD1 homology arms (GATAD1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HRPT2 L64P
Plasmid#11052DepositorInsertHRPT2 L64P (CDC73 Human)
UseTagsFlagExpressionMammalianMutationL64PPromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
OXGR1 -Tango
Plasmid#66466PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertOXGR1 (OXGR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
B9L-BirA*
Plasmid#109146PurposeTo generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertB9L (Bcl9l Mouse)
UseTagsBirA(R118G) and FlagExpressionMammalianMutationPromoterCMV/TetO2Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HDAC4 3A
Plasmid#52978Purposeinsect expression of HDAC4 3A constitutive active mutantDepositorInsertHDAC4 (HDAC4 Fly)
UseTagsFLAGExpressionInsectMutationS239,573,748A constitutively active*PromoterHsp70Available sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-plk4
Plasmid#136837PurposeMammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tagDepositorInsertCLIP-plk4 (PLK4 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2 TetO(sh) mCh-Rs1-2
Plasmid#24417DepositorInsertRs1
UseEntry vectorTagsFLAG and mCherryExpressionMutationHuman 5HT4B receptor with D100A mutation, generat…PromoterAvailable sinceMarch 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-CaMKKbeta S129A, S133A, S137A
Plasmid#32454DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseTagsFLAG and GFPExpressionMammalianMutationS129A, S133A, S137APromoterCMVAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pFETCh_KLF9
Plasmid#86286PurposeDonor vector for 3' FLAG tag of human KLF9DepositorInsertKLF9 homology arms (KLF9 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TAL-NC2
Plasmid#43855DepositorInsertTALEN-FokI nuclease backbone
UseTagsFlag and FokI nucleaseExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-chimpTRIM5-FOS
Plasmid#79065PurposeExpress chimpanzee TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(chimpanzee)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXB6-NA-MSCV
Plasmid#20992DepositorInsertHOXB6 (HOXB6 Human)
UseRetroviralTagsFlagExpressionMammalianMutationN51NPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
UseTags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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p5A7-T
Plasmid#92020PurposeExpresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with N and C-terminal TEV protease cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
hFlt1 V767A
Plasmid#86038Purposehuman VEGFR1 with V to A mutation at position 767DepositorInserthuman Flt1 V767A (FLT1 Human)
UseTagsFLAG, HA (please see depositor comments below), a…ExpressionMammalianMutationV767APromoterCMVAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
NPBW1-Tango
Plasmid#66448PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertNPBW1 (NPBWR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
NPBW2-Tango
Plasmid#66449PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertNPBW2 (NPBWR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 5/FR/TO-Ubl4A K48R
Plasmid#61811Purposeexpress C-terminal Ubl4A K48R, for making the stable cell lineDepositorInsertUBL4A (UBL4A Human)
UseTagsFLAGExpressionMammalianMutationK48RPromoterCMVAvailable sinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pSLQ10625
Plasmid#239268PurposeExpresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neuronsDepositorInsertNES-dPspCas13b-EGFP-ABI
UseTagsFlag tag and V5 tagExpressionMammalianMutationH133A and H1058A in PspCas13bPromoterCAGAvailable sinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
nCaMK2rep-S603A
Plasmid#239625PurposeInactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.DepositorInsertPSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
UseCre/Lox and LentiviralTagsFlag and mycExpressionMammalianMutationChanged serine 603 to AlaninePromoterhSynAvailable sinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FHpHG_mTent5c_dCAT
Plasmid#221442PurposeTransfection into the cells for the reporter or qPCR assay.DepositorInsertTent5c_AA (dCAT) (Fam46c Mouse)
UseTagsFLAG-2xHA-(HRV 3C site)-6xHis-EGFPExpressionMammalianMutationD90A, D92A (GATctgGAT to GCCctgGCC)PromoterAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT16
Plasmid#223388PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT41
Plasmid#223413PurposeT-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants select.DepositorInsertZmUbi-ecTadA8e-SpRY-D10A-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT15
Plasmid#223387PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by 2x35s and the sgRNA was driven by AtU3 promoter.DepositorInsert2x35s-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT17
Plasmid#223389PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT23
Plasmid#223395PurposeT-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and sgRNA was driven by OsU3 promoter.DepositorInsertZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT26
Plasmid#223398PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT28
Plasmid#223400PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT29
Plasmid#223401PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT30
Plasmid#223402PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only
