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pCMVhPOP1_3Flag

Plasmid

#53968

Purpose

Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

POP1 (POP1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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mSin1 shRNA #2

Plasmid

#13484

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1 (MAPKAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only
pQCXIP-GFP-LmnB1

Plasmid

#164249

Purpose

retroviral plasmid for GFP-LmnB1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

LMNB1 (LMNB1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-eGFP-MEFV

Plasmid

#92103

Purpose

Expression in eukaryotic cells and generating stable cell lines

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only
TPI-PTC160

Plasmid

#130698

Purpose

Expresses TPI-PTC160 NMD reporter (It has a Premature termination codon (PTC) at 160th Amino acid)

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-PTC160 (TPI1 Human)

Expression

Mammalian

Mutation

PTC160

Promoter

Pcmv

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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MCU-mCherry

Plasmid

#232790

Purpose

Encodes an mCherry-tagged MCU/Mitochondrial Calcium Uniporter

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

Calcium uniporter protein, mitochondrial (MCU )

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-SEC61B-mCherry-FRB

Plasmid

#233349

Purpose

Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

SEC61B (SEC61B Human)
mCherry
FRB

Use

Retroviral

Expression

Mammalian

Mutation

T2098L mutant of FKBP-rapamycin binding domain of…

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-MCU

Plasmid

#31726

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

*

Promoter

None

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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p5070 pcDNA4c hBrd4 (1047-1362)

Plasmid

#14449

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1047-1362) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1047-1362 of Brd4 (the CTD)

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
mEGFP-N1-SAV1

Plasmid

#166476

Purpose

Expresses fusion of mEGFP and SAV1

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, SAV1 (SAV1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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CS2-Myc-hASUN

Plasmid

#47955

Purpose

Expression of tagged-human ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2013 Jul 31.

Insert

Asunder (INTS13 Human)

Tags

Myc

Expression

Mammalian

Promoter

Sp6

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
p5069 pcDNA4c hBrd4 (471-730)

Plasmid

#14448

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (471-730) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 471-730

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLEX-FHH-MTORKinaseDomain

Plasmid

#120708

Purpose

Expresses Flag-HA-6xHIS-Mtor Kinase Domain fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

MTOR Kinase Domain (MTOR Human)

Use

Lentiviral

Tags

Flag-HA-6xHIS

Expression

Mammalian

Mutation

Amino acids 1967-2549

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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p5079 pcDNA4c hBrd4 (731-1046)

Plasmid

#14455

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (731-1046) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 731-1046

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLENTI-CMV-GFP-TREX1 delta C (aa1-235) BLAST

Plasmid

#164229

Purpose

lentiviral plasmid for GFP-hTREX1 delta C (aa1-235)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 235-314

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 IntS8 AA 1-308

Plasmid

#196905

Purpose

Expresses His-tagged Drosophila IntS8 amino acids 1-308

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

Integrator subunit 8 (IntS8 Fly)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pME GFP-PCNA

Plasmid

#105977

Purpose

entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

PCNA (PCNA Human)

Tags

GFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CIBN-GFP-TMD

Plasmid

#79571

Purpose

expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

syntaxin 1A (Stx1a Mouse)

Tags

CIBN and EGFP

Expression

Mammalian

Mutation

aa 249_288 (C-terminal region (polybasic stretch …

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2-OCRL(D523G)

Plasmid

#79567

Purpose

expression of CRY2PHR fused to phosphatase dead mutant of OCRL

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

OCRL (OCRL Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

aa 234_539 and phosphatase mutation, D523G

Promoter

CMV

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only