We narrowed to 23,588 results for: CHI

1. pGBW-m4046887

   Plasmid

   #145730

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pDD315 (mTurquoise2^SEC^2xHA)

   Plasmid

   #73343

   Purpose

   mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

   Insert

   mTurquoise2-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   2xHA and C. elegans codon-optimized mTurquoise2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 765 pME nls mCerulean

   Plasmid

   #195958

   Purpose

   Gateway pME fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean nuclear
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BAS286 C terminal SF-GFP hygBR

   Plasmid

   #74081

   Purpose

   S.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistanc

   Depositor

   Bassem Al-Sady

   Article

   Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

   Insert

   super-folder GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pWPI/hPLK2WT/Neo

   Plasmid

   #35385

   Depositor

   Anurag Tandon

   Article

   Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

   Insert

   hPLK2 (PLK2 Human)
   

   Use

   Lentiviral

   Tags

   EMCV IRES-Neomycin cassette and V5 epitope and Po…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   April 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME minprom_creERT2

   Plasmid

   #195966

   Purpose

   Gateway pME CreERT2 ORF

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mouse beta-globin minimal promoter creERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Opto-D2R

   Plasmid

   #106068

   Purpose

   Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-D2R
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 458 p3E-2A-EGFP-CAAXpA

   Plasmid

   #195968

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann, Kristen Kwan

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-EGFP-CAAX (membrane)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p5E-hs_ICAM_short (JDW 1046)

   Plasmid

   #224480

   Purpose

   Gateway compatible 5' entry clone containing the Human minimal ICAM2 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   min-ICAM2 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pREDKI

    Plasmid

    #51626

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRha

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GreenT-EC.b - Display - CyRFP1

    Plasmid

    #193944

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.b
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-Bmp4

    Plasmid

    #163588

    Purpose

    Expresses BMP-4 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp4 (Bmp4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQE60 R124K wgMDH

    Plasmid

    #204258

    Purpose

    Bacterial expression of R124K wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124K

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE60 R124Q wgMDH

    Plasmid

    #204259

    Purpose

    Bacterial expression of R124Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124Q

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE60 R124A wgMDH

    Plasmid

    #204257

    Purpose

    Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R124A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE60 R130E wgMDH

    Plasmid

    #204267

    Purpose

    Bacterial expression of R130E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130E

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME EFS-rtTA/rtTA3 (JDW 1230)

    Plasmid

    #224525

    Purpose

    A Gateway compatible middle entry clone containing a rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    EFS-rtTA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

    Plasmid

    #167898

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GreenT-EC.c - Display - CyRFP1

    Plasmid

    #193945

    Purpose

    Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

    Depositor

    Oliver Griesbeck

    Article

    Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

    Insert

    GreenT-EC.c
    

    Use

    Tags

    Igk_secretion - HA-Flag and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCIG2-Flag-mNuak1 WT

    Plasmid

    #168507

    Purpose

    For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    Nuak1 (Nuak1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTpPuc3

    Plasmid

    #62864

    Purpose

    Episome vector for Thalassiosira pseudonana (pUC-based)

    Depositor

    Hamilton Smith

    Article

    Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Episomal vector for thalassios…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA-flag-mKdm4d-polyA

    Plasmid

    #61553

    Purpose

    For mRNA synthesis or expression in mammalian cells

    Depositor

    Yi Zhang

    Article

    Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

    Insert

    Kdm4d (Kdm4d Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 459 p3E-2A-nlsEGFPpA

    Plasmid

    #195969

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-nlsEGFP (nuclear)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti c-MYC-DDK-Puro GFP

    Plasmid

    #123299

    Purpose

    Mammalian expression of GFP C-MYC-FLAG

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    MYC-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

    Plasmid

    #167902

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pirespuro2-CTLA4-mEos2

    Plasmid

    #98285

    Purpose

    mammalian expression of CTLA4-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Opto-GPR139

    Plasmid

    #106044

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR139; Opto-GPR139; D10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR139
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179119

    Purpose

    Expresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Ezr sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFETCh_RAD21

    Plasmid

    #64056

    Purpose

    Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RAD21 Homology Arms (RAD21 Human)
    

    Use

    Tags

    3X Flag P2A Neo

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKCcas9dO

    Plasmid

    #62552

    Purpose

    High efficiency Streptomyces genome editing by CRISPR/Cas9 system

    Depositor

    Weihong Jiang

    Article

    Huang et al Acta Biochim Biophys Sin (Shanghai). 201

    Inserts

    Scocas9
    sgRNA ()
    act-orf4 homology arm
    

    Use

    CRISPR; Bacterial genome editing

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    j23119 and ptipA

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-Bmpr2

    Plasmid

    #163403

    Purpose

    Expresses BMPR-2 in mammalican cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PresentER-MSIIFFLPL (GFP)

    Plasmid

    #102946

    Purpose

    PresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Insert

    MSIIFFLPL (Serpinf1 Synthetic, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLEX_970_puro_DEST_V5_SKIL

    Plasmid

    #45289

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pMXs-Lhx3

    Plasmid

    #32927

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Lhx3 (Lhx3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

37. YFP Gamma 8

    Plasmid

    #36106

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 8 (GNG8 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

38. p5E-0.349cldn15la

    Plasmid

    #125026

    Purpose

    Five prime entry vector containing 349bp cldn15la promoter from zebrafish. Drives expression in intestinal epithelial cells.

    Depositor

    John Rawls

    Article

    Murdoch et al PLoS Pathog. 2019 Mar 7;15(3):e1007381.

    Insert

    Claudin 15-like a (cldn15la Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDUAL_2_araBAD

    Plasmid

    #155176

    Purpose

    see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p5E TRE-3GV (JDW 1163)

    Plasmid

    #224476

    Purpose

    Gateway compatible 5' entry clone containing a 3rd generation Tet-On promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    TRE3GV promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. NAX_CAGGS_CAS9_m4_VPR_KanR neo

    Plasmid

    #167885

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pXSE901BG

    Plasmid

    #91714

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. Opto-M2R

    Plasmid

    #106070

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-M2R
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAS_FLAG-Mma PylRS

    Plasmid

    #154762

    Purpose

    plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. HiFi dCas9 VPR

    Plasmid

    #188510

    Purpose

    Expresses FLAG tagged HiFi dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSmac-GFP

    Plasmid

    #40881

    Depositor

    Douglas Green

    Article

    Tait et al Dev Cell. 2010 May 18;18(5):802-13.

    Insert

    Smac-GFP (DIABLO Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

49. pCK011 p5E exorh

    Plasmid

    #195949

    Purpose

    Gateway p5E vector

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh regulatory elements
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMXs-hu-N-Myc

    Plasmid

    #50772

    Purpose

    retroviral expression of human N-Myc

    Depositor

    Shinya Yamanaka

    Article

    Nakagawa et al Proc Natl Acad Sci U S A. 2010 Jul 26. (

    Insert

    N-myc (MYCN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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