We narrowed to 26,232 results for: Met;

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pCMV-DeAct-GS1

Plasmid

#89445

Purpose

Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-42)

Plasmid

#71875

Purpose

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-42) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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InsRA-eGFP

Plasmid

#79795

Purpose

Insulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Tags

eGFP extracellular

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Synthetic, Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has Service

AAV Retrograde, AAV1, and AAV5

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FuG-E

Plasmid

#67509

Purpose

This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

Depositor

Kazuto Kobayashi

Article

Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

Insert

Fusion Glycoprotein type E

Use

Lentiviral

Tags

Fusion protein of Vesicular stomatitis Indiana vi…

Promoter

CAGGS

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pTetON-DeAct-GS1

Plasmid

#89454

Purpose

Tet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

TRE3G

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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mEos3.2-Clathrin-15

Plasmid

#57452

Purpose

Localization: Clathrin Vesicles, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Article

Huang et al Nat Methods. 2013 Jul;10(7):653-8. doi:

Insert

Clathrin (CLTB Human)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

Plasmid

#55646

Purpose

Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp off chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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mRuby2-LaminA-C-18

Plasmid

#55901

Purpose

Localization: Nuclear Envelope, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LaminA (LMNA Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

Plasmid

#55648

Purpose

Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre off/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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M_ST1n_VPR

Plasmid

#63799

Purpose

ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; mammalian vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

ST1-dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

Plasmid

#84243

Purpose

Expresses ABA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sa dCas9
PYL1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

Plasmid

#47904

Purpose

An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

eNpHR3.0

Use

AAV

Tags

iRFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

nEF

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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