We narrowed to 25,508 results for: crispr
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Plasmid#207694PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pRU44
Plasmid#167666PurposeENTRY vector for gRNA4 cloning under pAtU3 promoterDepositorInsertpAtU3
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMZ283
Plasmid#52224PurposeExpression of sgRNA precursor with CspCI placeholder for target cloning (see comments).DepositorInsertrrk1-sgRNA
UseCRISPRExpressionYeastPromoterrrk1Available sinceOct. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9-CBP FL
Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBH532_MBP-Cas14b1 expression
Plasmid#112505PurposeExpresses 10xHis-MBP fused to Cas14b1 under t7 promoterDepositorInsertCas14b1
UseCRISPRTagsMBPExpressionBacterialPromoterT7Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-Actb KI #2
Plasmid#139666PurposeEndogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA and GFP donor
ExpressionMammalianPromoterU6 and CbhAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pDAC569
Plasmid#195241PurposeMammalian expression of Csm complex (RNase mut) from separate promoters; GFP backbone (Used for RNA binding/tethering)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3(RNase mut); FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
ExpressionMammalianAvailable sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXT131_TwinStrep-SUMO-ShTniQ
Plasmid#135527PurposeProtein expression construct for ShTniQDepositorInsertShTniQ
TagsTwinStrep-SUMOExpressionBacterialAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pXT129_TwinStrep-SUMO-ShTnsB
Plasmid#135525PurposeProtein expression construct for ShTnsBDepositorInsertShTnsB
TagsTwinStrep-SUMOExpressionBacterialAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BPK2301
Plasmid#65778PurposeHuman expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNADepositorInsertSt1Cas9 gRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPACE-NG (pRZ5150)
Plasmid#140244PurposeCMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSPACE_NG
ExpressionMammalianMutationV82G in TadA*, NG mutations in SpCas9, D10A in Sp…PromoterCMVAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p-dCas9-LSD1-Hygro
Plasmid#104406Purposetransient expression of dCas9-LSD1 fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSD1 (KDM1A Human)
UseCRISPRExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p-dCas9-JMJD2A-Hygro
Plasmid#104410Purposetransient expression of dCas9-JMJD2A fusion proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertJMJD2A (KDM4A Human)
UseCRISPRExpressionMammalianPromoterCMV promoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
SunTag-FWAgRNA-4-22aa-TET1cd
Plasmid#106435PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA-BfuAI-2k
Plasmid#112915PurposeEmpty sgRNA expression plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMB60
Plasmid#47941PurposeCan be used to generate synthetic guide in vitro from T7 promotorDepositorPublicationInsertsynthetic guide RNA
UseCRISPRExpressionWormAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCY5k
Plasmid#160294PurposeYeast CRISPR plasmid targeting the kanMX cassetteDepositorInsertssgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
URA3 selection cassette
sgRNA expression cassette (without guide)
Cas9
UseCRISPRTags8xHis-tag and SV40 NLSAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-TagRFP-t35s
Plasmid#118386PurposeEntry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p-TagRFP
UseCRISPRAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by