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pMXs-Sox2-17

Plasmid

#193353

Purpose

Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2-17 (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-W-FUGW

Plasmid

#166751

Purpose

Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a plasmid compatable for lentiviral infection. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Bcl-W (Bcl2l2 Mouse)

Use

Lentiviral

Tags

mCerulean3

Promoter

human ubiquitin C

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1K908E-VSV

Plasmid

#139357

Purpose

Expression of kinase-dead JAK1-K908E protein

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Tags

VSV

Expression

Mammalian

Mutation

K908E

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ULK2-K39I

Plasmid

#97076

Purpose

Expresses mutant ULK2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

ULK2 (ULK2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Lysine 39 to Isoleucine

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV N-term HA-FLAG puro BRD7 shRescue

Plasmid

#41936

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00642

Plasmid

#39706

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5748 (Hsf Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(1316-1538)

Plasmid

#35079

Depositor

Cheryl Arrowsmith

Article

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1316-1538

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00044

Plasmid

#34206

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17117 (hth Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
kiCAP-AAV-MyoAAV3F

Plasmid

#224447

Purpose

Rep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDHASW insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV3A

Plasmid

#224442

Purpose

Rep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYVGL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-pNFkB::Cre-mRuby3

Plasmid

#164137

Purpose

Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of TNFa

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

NFkB promoter-Cre-T2A-mRuby3

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

NFkB synthetic promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FB009

Plasmid

#119707

Purpose

Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC::nptII::Ttub

Use

Synthetic Biology

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00278

Plasmid

#34405

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9045 (Myb Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCALNL-Rheb(S16H)

Plasmid

#194204

Purpose

Cre/LoxP-dependent expression of a constitutively active form of Rheb (S16H). Expression of Cre excises a STOP codon before Rheb(S16H), facilitating expression of a constitutively active form of Rheb.

Depositor

Angelique Bordey

Article

Lin et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

Insert

Ras Homolog Enriched Protein in Brain (S16H) (RHEB Human)

Use

Cre/Lox

Expression

Mammalian

Mutation

S16H

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#163698

Purpose

AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

Depositor

Gina Turrigiano

Article

Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

Insert

CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mammalian

Promoter

CaMKII fragment

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pTn7·X-nia

Plasmid

#122592

Purpose

Plasmid for genomic integraction of xylS-Pm->nia into the Tn7 insertion site

Depositor

Pablo Ivan Nikel

Article

Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Insert

xylS/Pm->Nia

Use

Synthetic Biology; Tn7 genomic integration

Expression

Bacterial

Promoter

Pm

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTE4566

Plasmid

#88904

Purpose

Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1 deltains2

Plasmid

#78460

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2264-2315; Q2513R (see deposi…

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-mEKLF

Plasmid

#67833

Purpose

Expression of FL-EKLF driven by SV40 promoter

Depositor

James Bieker

Article

Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

EKLF (Klf1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-ERRbeta-delta10

Plasmid

#52187

Purpose

Expresses human ERRbeta-delta10 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only