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Plasmid#207077PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD3 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human SHLD3 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBsVchDonor
Plasmid#203814PurposeDNA donor for transpositionDepositorInsertMini-Tn, aprE cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM_ΔndhD2.km
Plasmid#185532Purposea pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locusDepositorInsertnptII gene flanked by ndhD2 upstream and downstream regions
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEM_Δflv3.sm
Plasmid#185533Purposea pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locusDepositorInsertaadA gene flanked by flv3 upstream and downstream regions
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-LoxP-sfGFP:ALFA
Plasmid#219660PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.DepositorInsertsfGFP:ALFA SEED Cassette (LoxP)
UseTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)
Plasmid#225441PurposeModule for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.DepositorInserthygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(0)
Plasmid#220446PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV100
Plasmid#215306PurposeExpresses sfGFP under induction with the 3OC6-HSL Marionette Cassette (LuxR + PLuxB-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPLuxBAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV110
Plasmid#215340PurposeExpresses sfGFP under induction with the Acrylic Acid Marionette Cassette (acuRAM + PAcu-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPAcuAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV107
Plasmid#215337PurposeExpresses sfGFP under induction with the DHBA Marionette Cassette (PcaUAM + P3B5B-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterP3B5BAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(2)
Plasmid#220448PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(1)
Plasmid#220447PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV105
Plasmid#215311PurposeExpresses sfGFP under induction with the choline Marionette Cassette (BetIAM + PBetI-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPBetIAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL391
Plasmid#219501PurposeExpress Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequenceDepositorInsertEco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV109
Plasmid#215339PurposeExpresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPCinAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV101
Plasmid#215307PurposeExpresses sfGFP under induction with the Vanillic Acid Marionette Cassette (VanRAM + PVanCC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPVanCCAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pITESceIcmd
Plasmid#218288PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1DepositorInsertHO(-253, -1)-tSynth3UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1
Plasmid#209321PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1 expression.DepositorInsertAPOBEC1-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rif1-Halo HRD
Plasmid#207081PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV111
Plasmid#215341PurposeExpresses sfGFP under induction with the Erythromycin Marionette Cassette (MphRAM + EryR + PMph-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPMphAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV106
Plasmid#215312PurposeExpresses sfGFP under induction with the naringenin Marionette Cassette (TtgRAM + PTtG-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialMutationPromoterPTtgAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Halo-SHLD2 HRD
Plasmid#207078PurposeHomologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-Sec61B
Plasmid#207553PurposeHomologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-Sec61B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SNAP-Sec61B
Plasmid#207580PurposeHomologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cellsDepositorInsertTET inducible SNAPTag-Sec61B
UseTagsSNAPTAGExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 LAMP1-mNeonGreen HRD
Plasmid#207549PurposeHomologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cellsDepositorInsertTET inducible LAMP1-mNeonGreen
UseTagsmNeonGreenExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-LC3B HRD
Plasmid#207551PurposeHomologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-LC3B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-GABARAPL1 HRD
Plasmid#207552PurposeHomologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-GABARAPL1
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
SNAP-LC3 HRD
Plasmid#207545PurposeHomologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cellsDepositorInsertSNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
UseTagsSNAPtagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only