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pcDNA3.1mLCAD_FLAG

Plasmid

#24484

Depositor

Eric Verdin

Article

Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

Insert

Long chain acyl-CoA dehydrogenase (Acadl Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
mCardinal-Tensin1-N-18

Plasmid

#56175

Purpose

Localization: Focal Adhesions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tensin1 (TNS1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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KRAS-P34R

Plasmid

#158527

Purpose

Expresses human KRAS P34R in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Article

Bera et al Birth Defects Res. 2020 Mar 18. doi: 10.

Insert

KRAS (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Promoter

T5

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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OR6K6_Deletion_gRNA2

Plasmid

#195196

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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OR6K6_Deletion_gRNA1

Plasmid

#195195

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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OR2M4_Deletion_gRNA

Plasmid

#195194

Purpose

dual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR2M4 dual gRNA (OR2M4 Human)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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OR6K6_Deletion_gRNA3

Plasmid

#195197

Purpose

dual gRNAs for deletion of OR6K6 in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

OR6K6 dual gRNA (OR6K6 Human)

Expression

Mammalian

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

Plasmid

#25948

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Sept. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3-ACSL4-FL

Plasmid

#202515

Purpose

Expressing luciferase driven by human ACSL4 promoter (bases -1 to -2844 with respect to exon1 start site)

Depositor

Ming Chen

Article

Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

Insert

ACSL4 promoter (partial) (ACSL4 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TUBG1

Plasmid

#171967

Purpose

pGEX2T-TUBG1 bacterial expression plasmid for production of recombinant GST fusion protein

Depositor

Maria Alvarado-Kristensson

Article

Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

Insert

TUBG1 (TUBG1 Human)

Tags

GST

Expression

Bacterial

Mutation

R275H (Please see depositor comments)

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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Human FLAG-WDR20

Plasmid

#217618

Purpose

Mammalian expression of FLAG tagged WDR20

Depositor

Ethan Lee

Article

Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

Insert

WDR20 (WDR20 Human)

Expression

Mammalian

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Ubac2

Plasmid

#123597

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

ubiquitin associated domain containing 2 (Ubac2 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114099

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-D358K

Plasmid

#91983

Purpose

Expresses FLAG-HA-AGO2 (D358K)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D358K

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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human a5 full length

Plasmid

#217818

Purpose

Full length human integrin a5 expression

Depositor

Timothy Springer

Article

Hao et al MAbs. 2024 Jan-Dec;16(1):2365891. doi: 1

Insert

ITGA5 (ITGA5 Human)

Tags

P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSRW1JZ

Plasmid

#129528

Purpose

C. elegans codon-optimized FlincG3 (GFP-based cGMP sensor) expressed in C. elegans ASE neuron pair

Depositor

Noelle L'Etoile

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgRNA-Scaffold-cTNT-SaCas9-HA-OLLAS

Plasmid

#209781

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA scaffold by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

cTNT

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-msCAMK2d-sgRNA-cTNT-SaCas9-HA-OLLAS

Plasmid

#209782

Purpose

Expresses SaCas9 by the specific cTNT promoter and sgRNA targeted the mouse Camk2d gene by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

sgRNA

Use

AAV

Tags

HA and OLLAS

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry-MKLP1

Plasmid

#140562

Purpose

Expresses mCherry tagged MKLP1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

MKLP1 (KIF23 Human)

Tags

2xmCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgA1

Plasmid

#187009

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgA1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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shYES1 # 2

Plasmid

#42547

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-Blast-sgCRTC2-1

Plasmid

#138700

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgCRTC2-2

Plasmid

#138701

Purpose

Expresses a human CRTC2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgCRTC2 human (CRTC2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
shYES1 # 1

Plasmid

#42546

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YES1 (YES1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
Lenti-EFS-hTSC1-T2A-Bsd

Plasmid

#193199

Purpose

Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

TSC1 (TSC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCD5-H5_Vietnam_1203/04_Y161A_sfGFP_ST

Plasmid

#182547

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus with a Y161A mutations conferring Neu5Gc specificity

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04_Y161A

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Y161A mutant that confers exclusive NeuAc to NeuG…

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114104

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CHAF1B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176969

Purpose

Mammalian expression vector encoding CHAF1B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CHAF1B (CHAF1B Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MKV937/nlp-1p::FlincG3

Plasmid

#140508

Purpose

This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

Depositor

Miri VanHoven

Article

Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

Insert

FlincG3

Expression

Worm

Promoter

nlp-1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-CES2A-ΔC-S227A

Plasmid

#200556

Purpose

Mammalian expression of secreted mouse CES2A S227A mutant

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-CES2A-ΔC

Plasmid

#200554

Purpose

Mammalian expression of secreted mouse CES2A

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-CD4

Plasmid

#175942

Purpose

knockout mouse CD4

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD4 gRNA (Cd4 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pNL-CEF-IgM

Plasmid

#187013

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgM

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mCardinal-Rab4a-7

Plasmid

#56174

Purpose

Localization: Ras GTPase, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Rab4a (RAB4A Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

Plasmid

#174134

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MDH1-miR-31SM-PGK-GFP-Blast

Plasmid

#31516

Depositor

Chang-Zheng Chen

Article

Arnold et al Genome Res. 2011 May . 21(5):798-810.

Insert

miR-31 (Mir31 Mouse)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

seed mutant control

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
BE3-hA3A-Y130F

Plasmid

#132945

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

BE3-hA3A(Y130F)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

BE3-hA3A(Y130F)

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Puro-sgMARK2

Plasmid

#138689

Purpose

Expresses a human MARK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK2 human (MARK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Puro-sgMARK3

Plasmid

#138690

Purpose

Expresses a human MARK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK3 human (MARK3 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 8641 - 8680 of 33014 results

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