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pSK221

Plasmid

#129249

Purpose

reverse yTRAP, tetR-responsive Neon reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

pADH1(tetO2)-mNeonGreen

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPaxillin-Electra2

Plasmid

#184926

Purpose

Expresses paxillin fused to blue fluorescent protein Electra2

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

paxillin-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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819 pGEX2T PTEN C124S

Plasmid

#10745

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

C124S

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pCas9-VRER_2A_GFP

Plasmid

#75475

Purpose

Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

Depositor

Marc Tessier-Lavigne

Article

Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

Insert

Cas9-VRER

Use

CRISPR

Tags

2A_GFP

Expression

Mammalian

Mutation

D1135V, G1218R, R1335E, T1337R

Promoter

CAG

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only

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817 pSG5L HA PTEN G129R

Plasmid

#10748

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

HA

Expression

Mammalian

Mutation

G129R

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pEThT-CAHS1

Plasmid

#90027

Purpose

To express His-tagged CAHS1 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMito-Electra1

Plasmid

#179481

Purpose

Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

COX8A-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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884 pGEX2T PTEN G129E

Plasmid

#10747

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

G129E

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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HA-RIPLET ΔRING

Plasmid

#218506

Purpose

RIPLET lacking N-terminal RING domain in pcDNA3.1

Depositor

Sun Hur

Article

Cadena et al Cell. 2019 May 16;177(5):1187-1200.e16.

Insert

RIPLET delta RING domain (RNF135 Human)

Expression

Mammalian

Available Since

May 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGAN147

Plasmid

#129202

Purpose

yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p6643 MSCV-P C-FlagHA 31E7-Kozak

Plasmid

#35021

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV31 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pEThT-SAHS2

Plasmid

#90026

Purpose

To express His-tagged SAHS2 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

SAHS2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-nbt-Electra1-P2A-mScarlet

Plasmid

#196113

Purpose

zebrafish expression of nbt-Electra1-P2A-mScarlet

Depositor

Reinhard Köster

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

nbt-Electra1-P2A-mScarlet

Use

Zebrafish expression

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pActin-Electra1

Plasmid

#184941

Purpose

beta-actin labelling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-beta-Actin

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCS-nlstdTomato

Plasmid

#132985

Purpose

full length of nuclear localized tdTomato in pCS expression vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

tdTomato

Tags

NLS and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCytERM-Electra1

Plasmid

#179476

Purpose

ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-GW

Plasmid

#194634

Purpose

Gateway destination vector for mammalian expression of Virotrap bait constructs

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

PQS1

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGAN213

Plasmid

#129270

Purpose

Control for measuring background levels of yTRAP reporter protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

mNeonGreen

Expression

Yeast

Promoter

min pCYC1

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pACEC3GH (Apr)

Plasmid

#110085

Purpose

Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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