We narrowed to 13,579 results for: cas9 genes

1. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA_tdtomato_reporter_1_SA-2x

   Plasmid

   #79369

   Purpose

   Assays activity of transcriptional activators fused to SA Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCPH2A.X_K5Q-GS3

   Plasmid

   #204746

   Purpose

   Expression of Cas9 and human H2AX with the K5Q mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K5Q (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K5Q mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCPH2A.X_K5R-GS3

   Plasmid

   #204747

   Purpose

   Expression of Cas9 and human H2AX with the K5R mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K5R (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K5R mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCPH2A.X_K134L-GS3

   Plasmid

   #204750

   Purpose

   Expression of Cas9 and human H2AX with the K134L mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K134L (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K134L mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCPH2A.X_K134M-GS3

   Plasmid

   #204751

   Purpose

   Expression of Cas9 and human H2AX with the K134M mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K134M (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K134M mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCPH2A.X_K134A-GS3

   Plasmid

   #204752

   Purpose

   Expression of Cas9 and human H2AX with the K134A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AX K134A (H2AX Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H2AX with the K134A mutation

   Promoter

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gh23

   Plasmid

   #106696

   Purpose

   expression of gRNA targeting POMT1, Cas9, and GFP

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   POMT1 (POMT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHvL1P4GA

   Plasmid

   #112028

   Purpose

   Expresses sgRNA in barley

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2019;1900:217-232. doi

   Insert

   TaU6-LacZ-sgRNA
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHvL1P3GA

    Plasmid

    #112027

    Purpose

    Expresses sgRNA in barley

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2019;1900:217-232. doi

    Insert

    LacZ
    

    Use

    Unspecified

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

    Plasmid

    #199496

    Purpose

    Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

    Depositor

    Robert Bryson-Richardson

    Article

    Miles et al bioRxiv 2023

    Insert

    Gal4vp16/4xnrUAS-mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSPble

    Plasmid

    #63561

    Purpose

    Bleomycin drug selection marker

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    Bleomycin drug selection marker
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. psBIG1a

    Plasmid

    #229957

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psBIG1b

    Plasmid

    #229958

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. psBIG2abc

    Plasmid

    #229963

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psBIG2ab

    Plasmid

    #229962

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psBIG2abcd

    Plasmid

    #229964

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psBIG2abcde

    Plasmid

    #229965

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psBIG1d

    Plasmid

    #229960

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psBIG1e

    Plasmid

    #229961

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. psBIG plasmids are recipient of multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge