We narrowed to 14,084 results for: cas9

1. pCPH2A-GS3

   Plasmid

   #204753

   Purpose

   Expression of Cas9 and human H2A

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2A (H2AC20 Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPH2A.J-GS3

   Plasmid

   #204757

   Purpose

   Expression of Cas9 and human H2A.J

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

   Insert

   H2AJ (H2AJ Human)
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX459-sg-tdTomato166/892

   Plasmid

   #179919

   Purpose

   Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   tdTomato sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJIT163-AFID-2

   Plasmid

   #167358

   Purpose

   For inducing predictable deletions in protoplasts and wheat plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG
   

   Expression

   Plant

   Mutation

   WT

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGM41991

   Plasmid

   #153219

   Purpose

   Level M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX300A-G+Nanog

   Plasmid

   #140280

   Purpose

   CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   Cas9
   

   Expression

   Mammalian

   Promoter

   CBH

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTX203

   Plasmid

   #89267

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsDEP1-gRNA02
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTX202

   Plasmid

   #89266

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsDEP1-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTX200

   Plasmid

   #89264

   Purpose

   Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01

   Depositor

   Daniel Voytas

   Article

   Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

   Insert

   OsYSA-gRNA01
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   Maize Ubiquitin1 promoter

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTX199

    Plasmid

    #89263

    Purpose

    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA02

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Insert

    OsPDS-gRNA02
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Promoter

    Maize Ubiquitin1 promoter

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSB700 Cerulean-Weissman scaffold

    Plasmid

    #79377

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB700 Cerulean-Zhang2.0

    Plasmid

    #79378

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    zhang 2.0
    

    Use

    CRISPR

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-dSa-VPR

    Plasmid

    #99651

    Purpose

    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9

    Depositor

    George Church

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VP64-p65-RTA activator

    Mutation

    dead Cas9

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lentiviral sgRNA human CD19

    Plasmid

    #155289

    Purpose

    Lentiviral expression of sgRNA against human CD19

    Depositor

    Martin Carroll

    Insert

    CD19 (CD19 Human)
    

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUDP002

    Plasmid

    #103872

    Purpose

    Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Inserts

    hph
    Spcas9 D147Y P411T
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Yeast

    Mutation

    D147Y P411T

    Promoter

    Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT3TS-zSpG

    Plasmid

    #179316

    Purpose

    In vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoter

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Zebrafish codon-optimized Cas9 variant SpG
    

    Use

    CRISPR; In vitro transcription by t3 promoter

    Tags

    SV40-NLS

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337R

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3TS-zSpRY

    Plasmid

    #179317

    Purpose

    In vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoter

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Zebrafish codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR; In vitro transcription by t3 promoter

    Tags

    SV40-NLS

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUDP123

    Plasmid

    #107269

    Purpose

    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. TLCV2

    Plasmid

    #87360

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Insert

    Cas9-2A-eGFP
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Tags

    Cas9-T2A-eGFP

    Expression

    Mammalian

    Promoter

    Tight TRE promoter

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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