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We narrowed to 2,225 results for: PAM

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Showing: 1051 - 1100 of 2225 results

  24. pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)

    Plasmid
    #233874
    Purpose
    Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)
    Tags
    His
    Expression
    Bacterial
    Available Since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233874-plasmid-map-sequence-id-467490

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  26. pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)

    Plasmid
    #233875
    Purpose
    Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification
    Depositor
    Emily Balskus
    Article
    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72
    Insert
    (+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)
    Tags
    His
    Expression
    Bacterial
    Available Since
    March 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233875-plasmid-map-sequence-id-463816

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  32. pcDNA3-CBX1mut-PA-mCherry

    Plasmid
    #138251
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Jin Zhang
    Article
    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:
    Insert
    CBX1mut-PAmCherry
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138251-plasmid-map-sequence-id-307435

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  40. pGMβ1

    Plasmid
    #216202
    Purpose
    Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.
    Depositor
    Yasuko Sasaki
    Article
    Iwamoto et al Biosci Microbiota Food Health. 2022;41(1
    Type
    Empty backbone
    Use
    Shuttle vector, conjugal plasmid, theta type rep…
    Expression
    Bacterial
    Promoter
    lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…
    Available Since
    Jan. 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    216202-plasmid-map-sequence-id-458484

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  42. pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

    Plasmid
    #191631
    Purpose
    E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS
    Depositor
    Emily Balskus
    Article
    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:
    Insert
    Dopamine dehydroxylase (R506S mutation, inactive form)
    Expression
    Bacterial
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    191631-plasmid-map-sequence-id-375966

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  47. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  50. pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

    Plasmid
    #169833
    Purpose
    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain
    Depositor
    Richard Possemato
    Article
    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422
    Insert
    CAD (CAD Human)
    Use
    Retroviral
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Deletions of amino acids R1398 - S1407 and F1366 …
    Available Since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    169833-plasmid-map-sequence-id-339176

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Showing: 1051 - 1100 of 2225 results