We narrowed to 615 results for: vp64

1. lentiSAMv2 OPTN TSS-guide1

   Plasmid

   #118196

   Purpose

   CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiSAMv2 BFP-guide1

   Plasmid

   #118165

   Purpose

   CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA1 against the BFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiSAMv2 OPTN TSS-guide3

   Plasmid

   #118198

   Purpose

   CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiSAMv2 OPTN TSS-guide2

   Plasmid

   #118197

   Purpose

   CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. lentiSAMv2 CAMK1D TSS-guide4

   Plasmid

   #118195

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiSAMv2 CAMK1D TSS-guide3

   Plasmid

   #118194

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. lentiSAMv2 CAMK1D TSS-guide1

   Plasmid

   #118192

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiSAMv2 EGFP-guide3

   Plasmid

   #118168

   Purpose

   CRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiSAMv2 CAMK1D TSS-guide2

   Plasmid

   #118193

   Purpose

   CRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a core and U6

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE EF1α dCas9-NED

    Plasmid

    #109369

    Purpose

    Lentiviral vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. sgRNA(MS2)_zeo insert c-MYC

    Plasmid

    #164636

    Purpose

    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64

    Depositor

    Günter Schneider

    Insert

    target sequence for c-Myc activation (MYC Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and EF1A

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LV anti-FITC-LaG16-Y186F/Y229A-2Xmutant-T2A-BFP

    Plasmid

    #194934

    Purpose

    LV construct encoding Y186F/Y229A double mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F/Y229A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

    Plasmid

    #101105

    Purpose

    Encodes dCas9(C) (without VP64) fused to VEGFR1

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR1-dCas9(C)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

    Plasmid

    #101114

    Purpose

    Encodes dCas9(N) fused to CXCR4

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    CXCR4-dCas9(C)-VP64
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVH15-Tier1-PhCMV-VPR

    Plasmid

    #169570

    Purpose

    Tier-1 vector encoding PhCMV-driven VP64-P65-Rta expression (PhCMV-VPR-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven VPR
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EC2_10_dCas9_VPR_HC_sgRNA

    Plasmid

    #163712

    Purpose

    Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LV-TRE-WT human MyoD-T2A-dsRedExpress2

    Plasmid

    #60628

    Purpose

    Expresses WT human MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    VP64 mouse MyoD (MYOD1 Human)
    

    Use

    Lentiviral

    Promoter

    TRE

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_501

    Plasmid

    #96922

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator VP64

    Depositor

    David Root, John Doench

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. V2-MESA-35F-M-dCas9

    Plasmid

    #84506

    Purpose

    MESA target chain with V2-MESA ectodomain, 35 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64

    Depositor

    Joshua Leonard

    Article

    Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

    Insert

    V2-MESA-35F-M-dCas9
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EC2_4_dCas9_VPR_sgRNA

    Plasmid

    #163709

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge