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ptyrEGFP-TYR

Plasmid

#32782

Depositor

Ruth Halaban

Article

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Insert

Tyrosinase (TYR Human)

Tags

EGFP

Expression

Mammalian

Promoter

Tyrosinase

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pCINeoFlag ALIX

Plasmid

#89859

Purpose

Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

ALIX (PDCD6IP Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

Plasmid

#92099

Purpose

Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR and TALEN; Zfn

Expression

Mammalian

Promoter

Tet-On 3G Bidirectional

Available Since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Article

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

alpha tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DNAseI

Plasmid

#182408

Purpose

Bacterial expression of DNaseI

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DNase I (DNASE1 Bovine)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K27

Plasmid

#22902

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K27 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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PB_iNEUROD1_P2A_GFP_Puro

Plasmid

#168803

Purpose

PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

NEUROD1 (NEUROD1 Human, Synthetic)

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4/Full length FVIII

Plasmid

#41036

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

FVIII (F8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6

Plasmid

#22900

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K6 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-ER-5

Plasmid

#55294

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

ER (CALR Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5-HA-Ubiquitin-K29

Plasmid

#22903

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K29 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-A3-HA-ubiquitin

Plasmid

#220145

Purpose

Expresses A3-Ub for E3 substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

Ubiquitin C (UBC Human)

Tags

A3-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO1

Plasmid

#53138

Purpose

E.coli expression of untagged mature human SUMO1 (amino acids 1-97)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-97)

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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XLone-ETV2

Plasmid

#135763

Purpose

expresses human ETV2 upon doxycycline treatment

Depositor

Xiaojun Lian

Article

Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

Insert

ETV2 (ETV2 Human)

Expression

Mammalian

Mutation

none

Promoter

TRE3G

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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p5068 pGEX-6P-1 Brd4 full-length

Plasmid

#14447

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (BRD4 Human)

Tags

GST

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K11

Plasmid

#22901

Depositor

Sandra Weller

Article

Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K11 only, all other lysines mutated to arginines

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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Rad51–Cas9(D10A)

Plasmid

#125567

Purpose

Expresses the Rad51–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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p-dCas9-SSAP-MS2-BB_BbsI

Plasmid

#183826

Purpose

pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

U6-guideRNA-dCas9-T2A-EBFP

Use

CRISPR

Expression

Mammalian

Promoter

CBH

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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ABCG1_pCSdest

Plasmid

#53952

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ABCG1 (ABCG1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pDARMO_CMVT_N_Strep_BIRC6

Plasmid

#197972

Purpose

Mammalian cell expression of wt Strep-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

Strep

Expression

Mammalian

Mutation

L1332V

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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