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TRPML1-HA

Plasmid

#18825

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML1 (MCOLN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Expression

Mammalian

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P05

Plasmid

#120016

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

eGFP

Use

Pcr template

Tags

eGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Cherry-Myo1b

Plasmid

#187366

Purpose

Expresses rat myosin 1b isoform b domain labelled with Cherry

Depositor

Evelyne Coudrier

Article

Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHIV-iRFP720-E2A-Luc

Plasmid

#104587

Purpose

Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

Depositor

Antonius Plagge

Article

Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

Insert

iRFP720-E2A-Luciferase

Use

Lentiviral and Luciferase

Tags

bi-cistronic

Expression

Mammalian

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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plk1 WT GFP-myc

Plasmid

#132963

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_WTTS

Plasmid

#91241

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEE393

Plasmid

#149283

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
pBstSK+IRES-V5-IPpoI

Plasmid

#206321

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag- IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX Nup62Fv2GFP

Plasmid

#139683

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-eKR2

Plasmid

#115337

Purpose

Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

Depositor

Peter Hegemann

Article

Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

Insert

eKR2

Tags

EYFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pmEGFP-N1

Plasmid

#217486

Purpose

Expression of monomeric Enhanced Green Fluorescent Protein

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_H2B-STf

Plasmid

#181998

Purpose

CMV overexpression of SNAP-tag fast fusion to H2B for nuclear localization in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

H2B-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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7xSMAD_RE::FLuc

Plasmid

#124531

Purpose

SMAD2 FLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

SMAD FLuc reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Red Glifon 3000

Plasmid

#163116

Purpose

Red fluorescent protein-based glucose sensor for mammalian cells with lower affinity

Depositor

Takashi Tsuboi

Article

Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

Insert

Red Glifon 3000

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_mCherry

Plasmid

#182939

Purpose

alpha7 nACHR expression in mammalian cell lines

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Use

Synthesized

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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