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pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain

Plasmid

#183397

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of human caspase-4 fused to catalytic domain of canine caspase-1/4

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-Luci

Plasmid

#31850

Depositor

Bruce Conklin

Article

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

Luciferase RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pST_SERF2_HUMAN (pBS0751)

Plasmid

#185239

Purpose

For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SERF2_HUMAN

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA (pBS0310)

Plasmid

#185169

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-SARS-CoV-2 SL1 alone-Luciferase

Plasmid

#191482

Purpose

Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

Depositor

Hao Wu

Article

Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

Insert

SARS-CoV-2 5' UTR SL1 alone

Use

Lentiviral and Luciferase

Mutation

Contains only the stem-loop1 region of SARS-CoV-2…

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Nanog-CreER targeting construct

Plasmid

#59720

Purpose

Cre-ER knockin targeting construct for mouse endogenous Nanog locus

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

creERT2

Use

Mouse Targeting

Promoter

mNanog

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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DsRed +9e

Plasmid

#191766

Purpose

Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of positively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pST_DHN_AtCor47 (pBS0784)

Plasmid

#185254

Purpose

For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_AtCor47

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDK197

Plasmid

#134189

Purpose

Retroviral plasmid expressing LAP-Clasp C terminus

Depositor

Iain Cheeseman

Article

Kern et al J Cell Biol. 2016 May 9;213(3):315-28. d

Insert

LAP-Clasp C terminus (CLASP1 Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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CKAP2 pHAT

Plasmid

#175946

Purpose

Bacterial expression of mmCKAP2 pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Susanne Bechstedt

Article

McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

Insert

CKAP2 (Ckap2 Mouse)

Tags

6-His and StrepTagII

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Gamma 5

Plasmid

#42200

Depositor

Narasimhan Gautam

Article

Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

Insert

Gamma 5 (Gng5 Rat)

Tags

Venus(1-155)

Expression

Mammalian

Mutation

codon-optimized

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pHelA12

Plasmid

#176493

Purpose

Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

Depositor

Xiaodong Cheng, Jian-Dong Huang

Article

Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

Insert

SARS-CoV NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

V570I

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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8200 Bicistronic_GFP_ires_hygro

Plasmid

#64375

Purpose

This a retroviral expression plasmid expressing GFP along with hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

EGFP

Use

Retroviral

Tags

EGFP

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

Plasmid

#160610

Purpose

Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:AsCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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R9-WT

Plasmid

#125210

Purpose

Expressing wildtype human Rad9b gene

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-Tyrosinase-bait-T2a-Cherry-pA

Plasmid

#111155

Purpose

knock in bait for Axolotl Tyrosinase locus

Depositor

Elly Tanaka

Article

Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

Insert

Tyrosinase-bait-T2a-Cherry-pA

Expression

Mammalian

Mutation

wt

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18

Plasmid

#172317

Purpose

CRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJS278

Plasmid

#134139

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255, R155A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCD5-ss-D/bovine/France/5920/2014-HEFs57aED-GCN4-sfGFP-ST

Plasmid

#175018

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

OK-HEF s57a ED

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

s57a esterase knock out mutant

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE0ΔiNLS

Plasmid

#107684

Purpose

Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE0ΔiNLS

Expression

Mammalian

Mutation

D832A/E925A/D1148A

Promoter

CMV

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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