We narrowed to 43,623 results for: CRISPR

1. pSC51

   Plasmid

   #104825

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

   Depositor

   Robert Stupar

   Article

   Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

   Insert

   Glyma.12g075700, Glyma.11g145900
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

   Plasmid

   #120300

   Purpose

   AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV CMV-driven AcrIIC1-2xmiR-122 target sites

   Plasmid

   #120302

   Purpose

   AAV vector for expression of AcrIIC1 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. AAV CMV-driven AcrIIC3-2xmiR-122 target sites

   Plasmid

   #120303

   Purpose

   AAV vector for expression of AcrIIC3 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC3-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

5. Lenti‐Cas9‐2A‐Blast

   Plasmid

   #73310

   Purpose

   Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDF-casBCDE(+6)

   Plasmid

   #89728

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a longer versi…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDF-casBCDE(-6)

   Plasmid

   #89730

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDF-casBCDE(-12)

   Plasmid

   #89731

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDF-casBCDE(-18)

   Plasmid

   #89732

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDF-casBCDE(-3)

    Plasmid

    #89729

    Purpose

    Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.

    Depositor

    Konstantin Kuznedelov, Konstantin Severinov

    Article

    Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    CRISPR array
    

    Expression

    Bacterial

    Mutation

    Derivative CRISPR array containing a shorter vers…

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRPCN

    Plasmid

    #193726

    Purpose

    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid

    #126881

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRAC10A

    Plasmid

    #193734

    Purpose

    Expresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion

    Depositor

    Martin Buck

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pICSL11086_GG_Wheat_live_Cas9_rev

    Plasmid

    #126882

    Purpose

    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pORFE1052

    Plasmid

    #112078

    Purpose

    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter

    Depositor

    Christian Chevalier

    Insert

    AtCAS9 D10A
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Plant codon optimized nCas9 D10A S. pyogenes

    Promoter

    2x35S

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pORFE0014

    Plasmid

    #112076

    Purpose

    AtCAS9 nickase D10A module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    AtCAS9 D10A without stop codon
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSIMcpf1

    Plasmid

    #153034

    Purpose

    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

    Insert

    Ascpf1
    

    Expression

    Bacterial

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P4 cosmid

    Plasmid

    #196335

    Purpose

    P4 cosmid with CRISPR-Cas9 system

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    genes from phage P4, CRISPR-Cas9 system
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Eef1a-1955/-1>nls::Cas9::nls

    Plasmid

    #59987

    Purpose

    Eef1a (EF1alpha) promoter driving nls::Cas9::nls

    Depositor

    Lionel Christiaen

    Article

    Stolfi et al Development. 2014 Nov;141(21):4115-20. d

    Insert

    nls::Cas9::nls
    

    Use

    CRISPR

    Mutation

    Reverted mutations from dCas9 to wiltdtype

    Promoter

    Ciinte.Eef1a (EF1alpha) -1955/-1

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge