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OPEN HLA-A*02:01-BirA

Plasmid

#215569

Purpose

E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN HLA-A*02:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

Glycine 120 to Cysteine

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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HF-PX459 (V2)

Plasmid

#118632

Purpose

Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

Depositor

Mike McGrew

Article

Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

Insert

hSpCas9-HF1-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Asparagine 497 to Alanine, Arginine 661 to Alanin…

Promoter

Cbh

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

Plasmid

#138148

Purpose

Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NES

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Lamp2b-HA

Plasmid

#71292

Purpose

Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

Flag, HA, and Lamp2 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

Plasmid

#195865

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2Cre

Plasmid

#82415

Purpose

Lentiviral vector expressing Cre recombinase alongside Cas9 and an sgRNA cloning site

Depositor

David Feldser

Article

Walter et al Cancer Res. 2017 Feb 15. pii: canres.215

Insert

Cre Recombinase

Use

CRISPR, Cre/Lox, and Lentiviral

Mutation

Mutated BsmBI cut site

Promoter

EFS (P2A)

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

Plasmid

#246430

Purpose

All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.

Depositor

Ailong Ke

Article

Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.

Inserts

O.gue IscB with dead mutations (D60A, H269A) and delta-TID
mCherry
ωRNA

Tags

SV40 NLS, Thosea asigna virus 2A peptide, and nuc…

Expression

Mammalian

Mutation

changed Aspartic Acid 60 to Alanine, changed Hist…

Promoter

CMV and U6

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pY108 (lenti-AsCpf1)

Plasmid

#84739

Purpose

Lenti virus delivery of AsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLX307-CRBN-3F-BirA

Plasmid

#220146

Purpose

Expresses CRBN-BirA for CRBN substrate identification by E-STUB

Depositor

William Sellers

Article

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

Cereblon (CRBN Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Promoter

EF-1a

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin1.OxLight1

Plasmid

#169792

Purpose

Fluorescent reporter for orexin neuropeptides

Depositor

Tommaso Patriarchi

Article

Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

Insert

OxLight1

Use

AAV

Promoter

human Synapsin-1

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

Plasmid

#201683

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEJS2602_AAV-Nme2-ABE8e-i1(V106W)-U6-2xSapI

Plasmid

#201684

Purpose

All-in-one AAV plasmid expressing Nme2-ABE8e-i1(V106W) and U6 sgRNA cassette. SapI enables cloning new spacers.

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i1 (V106W)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pUB-HspINTphiC31

Plasmid

#127518

Purpose

Plasmid encodes H. sapiens codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for H. sapiens expression.

Expression

Mammalian

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pX459-Reck gRNA

Plasmid

#246546

Purpose

CRISPR vector co-expressing Cas9 and a mouse Reck gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Reck gRNA (Reck Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Gpr124 gRNA

Plasmid

#246547

Purpose

CRISPR vector co-expressing Cas9 and a mouse Gpr124 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Gpr124 gRNA (Adgra2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-CA-mDia1

Plasmid

#45583

Depositor

Klaus Hahn, Ronen Zaidel-Bar

Article

Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

Insert

Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Insert mDia1 contains mutations V161D and N165D i…

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1P CISD1_1

Plasmid

#160773

Purpose

Suppress CISD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD1_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD1_2

Plasmid

#160774

Purpose

Suppress CISD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRF+423Dux4

Plasmid

#21625

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

Double homeobox, chr 4 (DUX4 Human)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only