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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,553 results for: pcas

Showing: 4461 - 4480 of 6553 results

  1. pDGB3 alpha1 P35S:BFP:T35S (GB3416)

    Plasmid
    #160637
    Purpose
    TU for the constitutive expression of Blue Fluorescent Protein
    Depositor
    Diego Orzaez
    Insert
    BFP
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160637-plasmid-map-sequence-id-321961

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  2. pRR2

    Plasmid
    #166097
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166097-plasmid-map-sequence-id-329109

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  3. pITI1

    Plasmid
    #166094
    Purpose
    Gal inducible expression of dCas9-FokI
    Depositor
    Rebecca Burgess
    Insert
    dCas9-FokI
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    spCas9-D10A, H840A
    Promoter
    Gal-inducible
    Available Since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166094-plasmid-map-sequence-id-329610

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  4. pEGB3_alpha1 Pnos:P19:Tnos (GB2762)

    Plasmid
    #160632
    Purpose
    TU for expression of P19 driven by Nos promoter.
    Depositor
    Diego Orzaez
    Insert
    P19
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160632-plasmid-map-sequence-id-321966

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  5. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  6. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

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  7. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196714-plasmid-map-sequence-id-388043

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  8. pAAVS1-CAG-hCAS9-neo

    Plasmid
    #166026
    Purpose
    AAVS1 targeting vector carrying CAG-SpCas9-neo
    Depositor
    Kosuke Yusa
    Insert
    AAVS1-CAGCas9IRESneo
    Expression
    Mammalian
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    166026-plasmid-map-sequence-id-328056

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  9. pLXIN2-RFP

    Plasmid
    #99205
    Purpose
    RFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    Red fluorescent protein
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Expression
    Mammalian
    Mutation
    The dsRed cDNA was PCR amplified off AddGene plas…
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99205-plasmid-map-sequence-id-192441

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  10. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  11. CaSpeR dhd 3X FLAG

    Plasmid
    #113002
    Purpose
    P element vector for transposon insertion of Flag-tagged Drosophila dhd into Drosophila genome
    Depositor
    Terry Orr-Weaver
    Insert
    dhd (dhd Fly)
    Use
    P insertion vector
    Tags
    FLAG
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    113002-plasmid-map-sequence-id-217654

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  12. Ring1-KO

    Plasmid
    #246224
    Purpose
    Knock out of Ring1
    Depositor
    Hengbin Wang
    Insert
    Ring1 gRNA (RING1 Human)
    Use
    Lentiviral
    Available Since
    Oct. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    246224-plasmid-map-sequence-id-487567

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  13. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  14. AtS/MAR10 insulator sequence (GB3459)

    Plasmid
    #160639
    Purpose
    pEGB3a2 AtS/MAR10
    Depositor
    Diego Orzaez
    Insert
    AtS/MAR10 insulator sequence
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160639-plasmid-map-sequence-id-321955

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  15. C2 H5 png-Ha/His in Casper

    Plasmid
    #112969
    Purpose
    P element vector for transposon insertion of png-HA/His into Drosophila genome
    Depositor
    Terry Orr-Weaver
    Insert
    png-HA/His (png Fly)
    Use
    P insertion vector
    Tags
    HA/His epitope
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112969-plasmid-map-sequence-id-217662

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  18. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid
    #160629
    Purpose
    TU for the expression of LbCas12a
    Depositor
    Diego Orzaez
    Insert
    AtUBQ10-:LbCpf1(Cas12a):Tnos
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    UBQ10
    Available Since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160629-plasmid-map-sequence-id-321990

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  19. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    Tet-inducible
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

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Showing: 4461 - 4480 of 6553 results