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Tol2-LyzC-RacFRET-U6ac-ctrl guides

Plasmid

#168247

Purpose

"label active Rac; control for neutrophil-specific knockout"

Depositor

Qing Deng

Article

Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

Insert

RacFRET

Use

Zebrafish expression

Promoter

LyzC

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shFmnl2-EGFP

Plasmid

#187255

Purpose

Lentiviral expression of shRNA targeting Fmnl2

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Fmnl2 (Fmnl2 Mouse)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2

Plasmid

#17793

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A

Plasmid

#42044

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

FLAG, HA, and SBP

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(925-1709)

Plasmid

#42004

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa925-1709

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

Plasmid

#211904

Purpose

Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

CD19-CAR-28z-2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

hPGK1

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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Rescue Septin 11 mRNA

Plasmid

#122022

Purpose

Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (AUU…

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pTracer_Cr-TRP1

Plasmid

#64881

Purpose

Expresses CrTRP1 in mammalian cells

Depositor

Sebastián Brauchi

Article

Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

Insert

Transient receptor potential 1

Tags

eGFP

Promoter

CMV

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
shTBX5 # 1

Plasmid

#42549

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shTBX5 (TBX5 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
KTK_002

Plasmid

#180527

Purpose

Entry vector containing J23104 promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

J23104 promoter

Expression

Bacterial

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pKG139

Plasmid

#69755

Purpose

Retroviral expression of CENP-C

Depositor

Iain Cheeseman

Article

Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

Insert

CENP-C (CENPC Human)

Use

Retroviral

Tags

mCherry-TEV-S

Expression

Mammalian

Mutation

L341F, N570D relative to NP_001803.2 (numbering b…

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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FRAP

Plasmid

#1827

Depositor

Philip Leder

Article

Ornitz et al Mol Cell Biol 1992 Jan;12(1):240-7.

Insert

FR1 AP (Fgfr1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-(457-924)

Plasmid

#42006

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa457-924

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL

Plasmid

#65712

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL

Use

Luciferase

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-Bmi1_3

Plasmid

#36354

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
E42_gRunx1_gRNA2_dTet_NGFR

Plasmid

#189801

Purpose

Retroviral delivery of guide RNA against mouse Runx1

Depositor

Ellen Rothenberg

Article

Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

gRunx1_gRNA2 (Runx1 Mouse)

Use

Retroviral

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP713-N2-Frankenbody-Flag

Plasmid

#175291

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with iRFP713

Tags

iRFP713

Expression

Mammalian

Available Since

Dec. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CAG-DIO-CFL-SN-P2A-GCaMP6f

Plasmid

#181742

Purpose

AAV vector for expressing CFL-SN and GCaMP6f

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN-P2A-GCaMP6f

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD EGFP

Plasmid

#187267

Purpose

Lentiviral expression of shRNA targeting Cortactin

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

shRNA for Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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