163,297 results

1. pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1

   Plasmid

   #124651

   Purpose

   Soma-localized ChrimsonR for dense expression in excitatory neurons

   Depositor

   Christopher Harvey

   Article

   Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

   Has Service

   AAV9

   Insert

   ChrimsonR
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CamKIIa

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1761

   Plasmid

   #142843

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF24 (ZNF24 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBPGAL80Uw-6

   Plasmid

   #26236

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Aug 9. ():.

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Synapsin sHRPa-NRX in FSW lentiviral vector

   Plasmid

   #73147

   Purpose

   Large fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-NRX
   

   Use

   Lentiviral

   Tags

   2x HA tag and Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Tol2

   Plasmid

   #31823

   Depositor

   Stephen Ekker

   Article

   Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

   Insert

   Tol2 Transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR1326

   Plasmid

   #111131

   Purpose

   Expresses UBE2T.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   UBE2T (UBE2T Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. C-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58694

   Purpose

   Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Csplit Intein and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_BiCHATe27_C1V1_eYFP

   Plasmid

   #213831

   Purpose

   AAV construct expressing eYFP-tagged chimeric opsin variant C1V1 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_C1V1_eYFP

   Depositor

   Gordon Fishell

   Article

   Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

   Insert

   C1V1-eYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH86966::AtU6p::sgRNA_PDS

   Plasmid

   #46966

   Purpose

   Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter

   Depositor

   Sophien Kamoun

   Article

   Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

   Insert

   AtU6p::sgRNA_PDS
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tg(mpeg1:EGFP-Mmu.Rpl10a)

    Plasmid

    #128840

    Purpose

    RNA isolation from machrophages via TRAP

    Depositor

    Anna Huttenlocher

    Article

    Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

    Insert

    EGFP-L10a
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP VAMP7 (1-220)

    Plasmid

    #42316

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 (1-220) (VAMP7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. EBFP2-N1

    Plasmid

    #54595

    Purpose

    Localization: N1 Cloning Vector, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCR1163

    Plasmid

    #111099

    Purpose

    Expresses FANCD2_+_10068200.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    FANCD2 gRNA (FANCD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTn7-SCOUT11.Y

    Plasmid

    #201004

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-sYFP2, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    sYFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23100

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_TetON_mCherry_P2AT2A_GFP-nb-KS-FtoG

    Plasmid

    #238242

    Purpose

    For integration of mCherry_P2AT2A_GFP-nb-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-KS-FtoG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-RCaMP1h

    Plasmid

    #42874

    Purpose

    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

    Depositor

    Loren Looger

    Article

    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

    Insert

    Red Fluorescent Calcium binding Protein
    

    Use

    Tags

    EK (Enterokinase) Recognition Site, His Tag (6x),…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85008

    Purpose

    For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    EGFP-ires-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. tol2-mpeg1-mcherry

    Plasmid

    #58935

    Purpose

    labels macrophages with mcherry

    Depositor

    Anna Huttenlocher

    Insert

    mpeg1-mcherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    mpeg1

    Available since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CAGGS-EGFP-NLS-3XFLAG-V5

    Plasmid

    #196090

    Purpose

    Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    GFP
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGs

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. mCherry-UVRAG

    Plasmid

    #86743

    Purpose

    N-terminal mCherry-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

    Insert

    human UVRAG (UVRAG Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSpCas9(BB)-2A-mCherry

    Plasmid

    #197420

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p426MET25_sfpHluorin (MRV55)

    Plasmid

    #115697

    Purpose

    superfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    sfpHluorin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. PB533-42B3mutAC2-sfGFP

    Plasmid

    #186777

    Purpose

    RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

    Insert

    42B3mutAC2-scFv
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PHB2-mCherry

    Plasmid

    #232785

    Purpose

    Encodes an mCherry-tagged Prohibitin2/PHB2

    Depositor

    Giulia Bertolin

    Article

    Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

    Insert

    PHB2 (PHB2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SF-SON IRES Puro

    Plasmid

    #214402

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

    Plasmid

    #190794

    Purpose

    Template vector to amplify pieces for creating multiplex sgRNA-arrays.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA-scaffold only
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23117

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV_U6-ωRNA*_ISDra2-TnpB-mNG

    Plasmid

    #212968

    Purpose

    AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

    Depositor

    Chunlong Xu

    Article

    Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

    Inserts

    ISDra2-TnpB-T2A-mNG,
    ωRNA*
    

    Use

    AAV and CRISPR

    Tags

    NLS and NLS-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a, T7 and U6

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge