We narrowed to 4,940 results for: HRE
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Plasmid#140457PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2361_Tier3(SB)-rtTA-YPet_PuroR
Plasmid#169657PurposeTier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2363_Tier3(PB)-rtTA-YPet_PuroR
Plasmid#169659PurposeTier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPRPBSAAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D
Plasmid#146129PurposeInsect Expression of DmEDC3_102-330DepositorInsertDmEDC3_102-330 (Edc3 Fly)
ExpressionInsectMutationone silent mutation A456G and three mutations N16…Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CPH3509 (YCp LEU2 Rpb1 T1471A)
Plasmid#91812PurposeYeast expression of S. cerevisiae Rpb1 with a T1471A mutationDepositorInsertRPO21 (RPO21 Budding Yeast)
ExpressionYeastMutationchanged threonine 1471 to alaninePromoterEndogenous Rpb1 promoterAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-CD8a(7A)
Plasmid#162498PurposeExpresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.DepositorInsertCD8a with a mutated stem region (CD8A Human)
TagsGFPExpressionMammalianMutationThe five threonine and two serine residues are mu…Available SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Met133I, M1381I, M186I ELL2
Plasmid#127263PurposeFor in vitro translation of human ELL2 with Met133I, M1381I, M186IDepositorInsertELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationMutationThree Mets (133, 138, 186) to IleuPromoterT7Available SinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONRG_P4-P1R:TBA2p
Plasmid#128429PurposeGateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.DepositorInsertTESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
UseGateway promoter entry clonePromoterTESTA ABUNDANT 2Available SinceOct. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19_T790M_28bp
Plasmid#114721Purpose28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …Available SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19_4xT790M_28bp
Plasmid#114719Purpose4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standardDepositorInsertEGFR (EGFR Human)
ExpressionBacterialMutationc.2369C>T (Substitution, position 2369, C➞T); …Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
csd2_121-308,T151A/pET28b(+)
Plasmid#83300Purposecsd2, construct: 121-308, pET28b(+) NC-tag, mutation: T151ADepositorInserthp1544
TagsHis tagExpressionBacterialMutationdeleted amino acid 1-120, and changed Threonine 1…Available SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP64-hERG1a S620T
Plasmid#53059PurposeExpresses alpha subunit of S620T hERG1a K channel in Xenopus oocytesDepositorInsertPotassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
UseXenopus oocyte expressionMutationchanged Serine 620 to ThreoninePromoterSP6Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG MKK3b(Glu, delete LXL)
Plasmid#50452Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
TagsGstExpressionMammalianMutationdeleted L25-I27, changed Serine 218 to Glutamic A…PromoterSV40Available SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT-7th R
Plasmid#42573DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDYT001
Plasmid#186549PurposeLineage tracing vector (with barcoded Target Sites and triple sgRNAs)DepositorInsert14-bp random integration barcode and three target sites and 3x sgRNAs
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …Promoterelavl3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-AID-BubR1
Plasmid#47330Purposefor plasmid integration using the Flp-In system. AID at N-terminalDepositorInsertBUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
TagsAID and GFPExpressionMammalianMutationsiRNA resistantAvailable SinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEB1deltaC-mCherry
Plasmid#80603PurposeExpresses C-terminally truncated, mCherry-tagged EB1 as marker for growing microtubule plus endDepositorInsertEB1 (MAPRE1 Human)
TagsmCherryExpressionMammalianMutationTruncated C-terminus; EB1 coding sequence ends at…PromoterCMVAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS
Plasmid#154774Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
TagsFLAGExpressionMammalianMutationin PylT: UUA anticodon and G10A, G14U, G16U, C20U…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-muhCD4
Plasmid#162745PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
UseRetroviralExpressionMammalianMutationHuman CD4 gene with glutamine to tyrosine at posi…Available SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only