315,891 results

1. pFastbac1-Flag-FANCD2

   Plasmid

   #134904

   Purpose

   codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   FANCD2 (FANCD2 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   polyhedron

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastbac1-Flag-FANCD2

   Plasmid

   #134904

   Purpose

   codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   FANCD2 (FANCD2 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   polyhedron

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176269

   Purpose

   Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCasMINI-V4-VPR-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176269

   Purpose

   Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCasMINI-V4-VPR-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenLox_U6 p53

   Plasmid

   #59360

   Purpose

   Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

   Depositor

   Peter Scheiffele

   Article

   Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

   Inserts

   shRNA p53 (mouse) (Trp53 Mouse)
   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and mouse U6

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenLox_U6 p53

   Plasmid

   #59360

   Purpose

   Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

   Depositor

   Peter Scheiffele

   Article

   Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

   Inserts

   shRNA p53 (mouse) (Trp53 Mouse)
   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and mouse U6

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209651

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eGFP-SUN1

   Plasmid

   #213515

   Purpose

   Expresses human eGFP-SUN1 in mammalian cells

   Depositor

   Karim Mekhail

   Article

   Shokrollahi et al bioRxiv 2023.05.07.539750

   Insert

   SUN1 (SUN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SUN1 has a silent mutation on its gene (nucleotid…

   Promoter

   CMV

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. eGFP-SUN1

    Plasmid

    #213515

    Purpose

    Expresses human eGFP-SUN1 in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    SUN1 (SUN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1 has a silent mutation on its gene (nucleotid…

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225242

    Purpose

    Mammalian expression of iPASnFR-L in the cytosol

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Use

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. M7 pdnPKA-GFP

    Plasmid

    #16716

    Depositor

    Randall Moon

    Article

    Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

    Insert

    dnPKA (Prkar1a Mouse)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Dominant negative. Mutations at cAMP binding site…

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. M7 pdnPKA-GFP

    Plasmid

    #16716

    Depositor

    Randall Moon

    Article

    Ungar et al Dev Biol. 1996 Aug 25;178(1):186-91. doi

    Insert

    dnPKA (Prkar1a Mouse)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Dominant negative. Mutations at cAMP binding site…

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUW-TetON-Sox10

    Plasmid

    #115242

    Purpose

    Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-TetON-Sox10

    Plasmid

    #115242

    Purpose

    Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

    Plasmid

    #188777

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mNeonGreen-KDEL

    Plasmid

    #226568

    Purpose

    Encodes mNeonGreen with KDEL ER lumenal target sequence

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    KDEL (ER lumenal target sequence)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-NPM1wt-IRES-mCherry

    Plasmid

    #131824

    Purpose

    Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLVX-NPM1wt-IRES-mCherry

    Plasmid

    #131824

    Purpose

    Lentiviral plasmid expressing NPM1 wildtype (human) in mammalian cells upon transduction

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pET28a-At-TRL

    Plasmid

    #32242

    Depositor

    Stan Fields, Jay Hesselberth

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    A. thaliana tRNA ligase (RNL Mustard Weed)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polmyerase

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-At-TRL

    Plasmid

    #32242

    Depositor

    Jay Hesselberth, Stan Fields

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    A. thaliana tRNA ligase (RNL Mustard Weed)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polmyerase

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-ccdB-V5

    Plasmid

    #214985

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1-ccdB-V5

    Plasmid

    #214985

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-hISG15

    Plasmid

    #12447

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15 fragment (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ISG15 up to LRLRGG

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-hISG15

    Plasmid

    #12447

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    ISG15 fragment (ISG15 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ISG15 up to LRLRGG

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge