We narrowed to 3,510 results for: Braf;

1. pCrysβ:ECFP-LexOP:mCherry-HrasG12V

   Plasmid

   #89887

   Purpose

   Human HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   mCherry-HRasG12V
   

   Use

   Unspecified

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124431

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Zebrafish (if expression level is too high in zeb…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-gfap-sgRNA

   Plasmid

   #65566

   Purpose

   in vitro trancription of sgRNA targeting the zebrafish gfap locus

   Depositor

   Jiu-lin Du

   Article

   Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

   Insert

   gfap sgRNA (gfap Zebrafish)
   

   Use

   In vitro rna transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168242

   Purpose

   "Rescue Rac expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-WT
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-CMV-zdgat2FLAG-SV40

   Plasmid

   #232141

   Purpose

   Expresses zebrafish dgat2 with a C-terminal 3xFLAG tag under control of the CMV promoter

   Depositor

   Steven Farber

   Article

   Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

   Insert

   dgat2 (dgat2 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-LyzC-Rac-DN(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168243

   Purpose

   "Dominant negative control of the assay to rescue Rac expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-DN
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides

   Plasmid

   #168244

   Purpose

   "Rescue Rac-CA expression in neutrophil specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   Rac(guide resistant)-CA
   

   Use

   Zebrafish expression

   Tags

   mcherry

   Expression

   Mutation

   Promoter

   LyzC

   Available since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2-zfKitlga

   Plasmid

   #168199

   Purpose

   Express zebrafish Kitlga, mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

   Insert

   Kitlga (kitlga Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Tags

   Expression

   Mammalian

   Mutation

   F198L - please see depositor comments

   Promoter

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUFlip-floxed2A-mRFP-; HE1A:BFP -2

   Plasmid

   #180009

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUFlip-floxed2A-mRFP-; HE1A:BFP -0

    Plasmid

    #180007

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUFlip-floxed2A-mRFP-; HE1A:BFP -1

    Plasmid

    #180008

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

    Plasmid

    #180010

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

    Plasmid

    #180011

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUFlip-floxed2A-KalTA4-; HE1A:BFP -2

    Plasmid

    #180012

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-KalTA4; HE1A: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUFlip-floxed2A-mRFP-; gcry1:BFP -2

    Plasmid

    #173888

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUFlip-floxed2A-mRFP-; gcry1:BFP -0

    Plasmid

    #173886

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUFlip-floxed2A-mRFP-; gcry1:BFP -1

    Plasmid

    #173887

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSYC-437

    Plasmid

    #163829

    Purpose

    Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

    Depositor

    Seok-Yong Choi

    Article

    Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

    Inserts

    Foxj1a Promoter
    GCaMP6s
    

    Use

    Multisite gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

    Plasmid

    #118845

    Purpose

    Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    ptena and ptenb (ptena, ptenb Zebrafish)
    

    Use

    CRISPR; Tol2

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-zfIl34

    Plasmid

    #168112

    Purpose

    Express zebrafish Il34, mRNA synthesis

    Depositor

    Petr Bartunek

    Article

    Hason et al Blood Adv. 2022 Jan 3. pii: 483305. doi:

    Insert

    Il34 (il34 Zebrafish)
    

    Use

    Vertebrate expression (zebrafish, chicken, xenopu…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge