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pAFM-NbrWT

Plasmid

#40830

Purpose

Expresses Flag, Myc tagged Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
gRD21A-mRFP

Plasmid

#181959

Purpose

RD21A protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

RD21A genomic sequence including 2kb upstream of the ATG

Tags

mRFP

Expression

Plant

Promoter

RD21A native

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human APC-B,C (1133–1189)

Plasmid

#132630

Purpose

expresses human APC-B,C (1133–1189) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

Insert

APC-B,C (1133–1189)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

Dog Cav1-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCambia1300-ba-ar

Plasmid

#226815

Purpose

Efficient screening of gene editing in plants (Basta resistance).

Depositor

Daisuke Miki

Article

Kong et al Hortic Res. 2025 Nov 5;12(11):uhaf196. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCambia1300-NP-PTII

Plasmid

#226816

Purpose

Efficient screening of gene editing in plants (kanamycin resistance).

Depositor

Daisuke Miki

Article

Kong et al Hortic Res. 2025 Nov 5;12(11):uhaf196. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-P2A-Puro

Plasmid

#211645

Purpose

EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF226_EFS-Cas9-P2A-Puro

Plasmid

#211644

Purpose

EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-IC2N-dCer3

Plasmid

#191325

Purpose

Chronically inhibit dynein by expressing the dominant negative acting N-terminal domain of the dynein intermediate chain (IC2N)

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

IC2N-dCer-FRB (Dync1i2 Mouse)

Expression

Mammalian

Mutation

aa 1-237

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-MAFB-sg

Plasmid

#194718

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (MAFB Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S7-S83C/Δ157-178

Plasmid

#128592

Purpose

Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

S7 E.coli ribosomal protein (rpsG E.coli)

Expression

Bacterial

Mutation

S83C, Δ157-178

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-CfANLN_sgRNA

Plasmid

#183880

Purpose

pX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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gMUM2-Citrine

Plasmid

#181965

Purpose

MUM2 protein fused to Citrine, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

MUM2 (MUM2 Mustard Weed)

Tags

Citrine YFP

Expression

Plant

Promoter

MUM2

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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MUM2SP-Citrine

Plasmid

#181963

Purpose

MUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

MUM2 signal peptide, fused to the Citrine YFP in pAD

Tags

Citrine YFP

Expression

Plant

Promoter

MUM4pro 1.5kb fragment

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTL316

Plasmid

#168377

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

LOC_ Os08g40130

Expression

Yeast

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTL360

Plasmid

#168421

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

LOC_ Os01g70240

Expression

Yeast

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTL362

Plasmid

#168423

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

LOC_ Os04g32860

Expression

Yeast

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTL374

Plasmid

#168435

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

LOC_ Os02g48170

Expression

Yeast

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTL387

Plasmid

#168448

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

LOC_ Os04g39370

Expression

Yeast

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTL394

Plasmid

#168455

Purpose

pGBKT7 yeast two-hybrid plasmid

Depositor

Sophien Kamoun

Article

Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

Insert

LOC_ Os03g02070

Expression

Yeast

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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