We narrowed to 21,997 results for: LEA

Showing: 4581 - 4600 of 21997 results

Results per page:

Sort results by:

FUG-Pten

Plasmid

#140732

Purpose

Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Publication

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-Pten (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

hUbiC

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-actin

Plasmid

#118378

Purpose

Gateway compatible donor vector with WT actin.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

Plasmid

#86417

Purpose

luminal split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Publication

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-mCherry-SCS2TM-GFP11

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pRRL_U2AF1_S34Y_mCherry

Plasmid

#84018

Purpose

expression of mutant U2AF1 in mammalian cells

Depositor

Robert Bradley

Publication

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Mutation

S34Y

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
OCT4 GFP puro Donor 3

Plasmid

#22211

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Publication

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Promoter

T7

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA_B2AR-no-pep

Plasmid

#47439

Purpose

control FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Publication

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pET28-SpRY-HF1-NLS-6xHis (gbr2102)

Plasmid

#181744

Purpose

bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag

Depositor

Benjamin Kleinstiver

Publication

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

bacterial codon optimized SpRY-HF1

Use

In vitro transcription; t7 promoter

Tags

BPNLS(SV40)-6xHis and MKIEE MBP tag

Expression

Bacterial

Mutation

SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…

Promoter

T7

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-FLAG-CXCR4

Plasmid

#98947

Purpose

Mammalian expression plasmid for N-terminal FLAG-tagged human CXCR4

Depositor

Erik Procko

Publication

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CXCR4 (CXCR4 Human)

Tags

FLAG (dykdddd) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO HSPH2

Plasmid

#19454

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pNH-NanoLuc

Plasmid

#173075

Purpose

Expression vector for E. coli producing target protein with N-terminal Nanoluciferase fusion

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pCDNA5_FRT_TO_TurboID-mCherry-Cry2

Plasmid

#166504

Purpose

Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

Depositor

Angelos Constantinou

Publication

Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

Insert

None

Tags

TurboID and mCherry-CRY2

Expression

Mammalian

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Publication

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HAP40 1-371

Plasmid

#124060

Purpose

Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

Depositor

Cheryl Arrowsmith

Publication

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HAP40 (F8A1 Human)

Use

Baculovirus expression

Tags

6x His and TEV cleavage site

Promoter

polyhedrin

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pcDNA3-ExRai-AktAR(T/A)

Plasmid

#118463

Purpose

Negative-control mutant for ExRai-AktAR biosensor.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AktAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIG-hNFATc1/bC

Plasmid

#74049

Purpose

retroviral expression plasmid for human NFATc1/bC

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

no tag

Expression

Mammalian

Promoter

pMSCV-LTRs

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
AAV-miniSOG-VAMP2-citrine

Plasmid

#50969

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

Depositor

Roger Tsien

Publication

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-citrine (Vamp2 Arabdopsis thaliana, Mouse)

Use

AAV

Tags

citrine and miniSOG

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti4/TO-hEPO

Plasmid

#50437

Purpose

express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system

Depositor

Juan Melero-Martin

Publication

Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

Insert

EPO (EPO Human)

Use

Lentiviral; Tetracycline-regulatable (t-rex)

Expression

Mammalian

Promoter

CMV/TO

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCS2+ macroH2A1.2-GFP-HA

Plasmid

#30515

Depositor

John Gurdon

Publication

Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

Insert

macroH2A1.2-C (Macroh2a1 Mouse)

Use

Xenopus/zebrafish expression.

Tags

eGFP and triple HA

Expression

Mammalian

Mutation

NO STOP

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pcDNA3.1 FLIPE-600n

Plasmid

#13541

Depositor

Wolf Frommer

Publication

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Mammalian

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge