We narrowed to 18,824 results for: nls
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Plasmid#82606PurposeExpresses sfCherry2(11) and TagBFP fusion in mammalian cellsDepositorInsertsfCherry2(11)_TagBFP
UseLentiviralExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterSFFVAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ220 (AsCpf1)
Plasmid#86208PurposeAsCpf1 Gateway entry plasmidDepositorInsertAsCpf1
UseGateway compatible cpf1 vectorsTags5' and 3' NLSExpressionPlantAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ233 (dLbCpf1-SRDX)
Plasmid#86211PurposeDeactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPRTags5' and 3' NLS and SRDX repressorExpressionPlantAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE2 mCherry-Atl3
Plasmid#86680PurposeLentivirus to stably express fluorescent protein in mammalian cellsDepositorAvailable sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML104-HygMx4
Plasmid#83475PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.DepositorInsertHphMX4
ExpressionYeastAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCas9-polyA
Plasmid#72602Purposeexpress hCas9 byT7 promoter with polyadenine tailsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9-polyadenine
Tags81-bp polyadenylationPromoterT7Available sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMpGE011
Plasmid#71537PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
spCas9-BlastR (pCBhCas9-BlastR)
Plasmid#71489PurposeExpresses SpCas9 and Blasticidin resistanceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9
PromoterCBhAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHSN501
Plasmid#50589PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertszCas9D10A
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG and NLSMutationCas9D10A nickase, was derived from the zCas9 and …Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
TALEN-mROSA26 ELD
Plasmid#60026PurposeExpression of a TALEN protein specific for mouse ROSA26 locus.DepositorInsertTALEN-ROSA26
UseTALENExpressionMammalianAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pThy1-Flpbow3
Plasmid#45181DepositorInsertpThy1-Flpbow3
UseLinearized for transgenic mouse injectionPromoterThy1Available sinceNov. 19, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC149-pCR8-dCas9VP160
Plasmid#48221PurposedCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9(D10A;H840A) fusion with VP160 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP160MutationD10A;H840A nuclease-deficientAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC91-pmax-dCas9VP64
Plasmid#48223PurposedCas9VP64 on pmax expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPRTagsHA Tag and VP64ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGal4AD-CIBN
Plasmid#28246PurposeExpresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activationDepositorInsertCIB1 (1-171aa)
TagsGal4ADExpressionYeastAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TET-mSox2
Plasmid#20908PurposepiggyBac vector with tet inducible mSox2DepositorAvailable sinceApril 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFPpA-ACN
Plasmid#20343DepositorPublicationInsertEGFPpA-ACN
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable sinceMarch 11, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVPT-GDNF-rtTR-KRAB-2SM2
Plasmid#11647PurposeTet-regulated (Tet-off) lentiviral vector for GDNF (mPGK promoter) - 2nd generationDepositorAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBUE411-GGB
Plasmid#198233PurposeWheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411DepositorPublicationInsertsGRF4-GIF1 chimera cassette
pPLTP::BBM cassette
UseCRISPR; Plant binary vectorExpressionPlantPromoterUbiquitin and maize PLTPAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only