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pEThT-MAHS delta MTP

Plasmid

#90032

Purpose

To express His-tagged MAHS in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Tags

His

Expression

Bacterial

Mutation

Deletion of mitochondria targeting peptide

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 MEKK3 WT

Plasmid

#12186

Depositor

Gary Johnson

Article

Blank et al J Biol Chem. 1996 Mar 8. 271(10):5361-8.

Insert

MEKK3 (Map3k3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pCDH-Lenti-CAGGS-hPRDM6-2xGly-V5-EF1-Luc2-Blast

Plasmid

#213826

Purpose

Expresses human PRDM6 with a C-terminal V5 epitope tag.

Depositor

Bjoern Schwer

Article

Schmidt et al bioRxiv. 2023 Aug 31:2023.08.29.555389.

Insert

PRDM6 (PRDM6 Human)

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Soma-jGCaMP8f

Plasmid

#169258

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis-tag

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pWF079_LVt-EF1a-TCRGibson_humanTRAC

Plasmid

#233448

Purpose

Gibson assembly of TCRB-P2A-TCRa

Depositor

William Freed-Pastor

Article

Ely et al Science. 2025. May 8; 388(6747)

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Article

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Insert

Axin (Axin1 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pDule-Acd82

Plasmid

#174099

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Article

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Promoter

hSyn

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Structural polyprotein

Plasmid

#215698

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pY31

Plasmid

#84746

Purpose

Expresses huLbCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Brn2.WPRE

Plasmid

#67294

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Brn2 (Pou3f2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Dnm2a

Plasmid

#109519

Purpose

Muscle specific zebrafish expression of mKate2 fused to Dynamin2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dnm2a (dnm2a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Actin-7

Plasmid

#57120

Purpose

Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Olenych et al Curr Protoc Cell Biol. 2007 Sep;Chapter

Insert

Actin (ACTB Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

Plasmid

#47457

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Tags

2A_GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pLv-CMV-MyoD

Plasmid

#26808

Depositor

Jeffrey Chamberlain

Article

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pQE32-pr-mEosFP (mEosFP-V69T)

Plasmid

#99213

Purpose

Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEosFP

Expression

Bacterial

Mutation

monomeric variant with mutation V69T for primed c…

Promoter

T5

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-NLGN2

Plasmid

#82545

Purpose

Overexpression vector for V5-NLGN2 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

NLGN2 (Nlgn2 Rat)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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