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pCS-lpp0.2-H38A

Plasmid

#201788

Purpose

mutant of PadR; transcriptional regulator can respond to p-coumaric acid

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PadR (padR Bacillus subtilis)

Expression

Bacterial

Mutation

H38A

Available Since

July 12, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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A1ACR1_EYFP_pcDNA3.1

Plasmid

#161025

Purpose

Expresses strongly red-shifted A1ACR1 in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Proc Natl Acad Sci U S A. 2020 Sep 15;11

Insert

A1ACR1

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-MCP1

Plasmid

#184341

Purpose

Express yeast MCP1 in Expi293F cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Yeast MCP1 (MCP1 Budding Yeast)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pX165-evoCas9

Plasmid

#140569

Purpose

Expresses evoCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

M495V Y515N K526E R661Q

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BREP

Plasmid

#172337

Purpose

BRET-Based Bioluminescent Red Protein

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-BREP

Plasmid

#172340

Purpose

AAV transfer plasmid for BREP (bioluminescent red protein)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BREP

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCTX-myc-HIPK2 WT

Plasmid

#32996

Depositor

Sergei Sokol

Article

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

HIPK2 (hipk2 Frog)

Tags

6XMyc

Promoter

SCMV

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pRGEB34

Plasmid

#158152

Purpose

Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pExp_TRE:EBFP2

Plasmid

#51793

Purpose

Inducible expression of EBFP2 with rtTA for input fluorescence

Depositor

Ron Weiss

Article

Davidsohn et al ACS Synth Biol. 2014 Nov 17.

Insert

EBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-TTLL11

Plasmid

#140582

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA TTLL11

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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JAF99.4

Plasmid

#45560

Depositor

Gregory Pazour

Article

Follit et al J Cell Biol. 2010 Jan 11;188(1):21-8. do

Insert

Pkhd1 (Pkhd1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains only the cytoplasmic tail (last 193 amin…

Promoter

CMV

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pU6-pegRNA-HEK3+24

Plasmid

#140577

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA HEK3 +24

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Rap2b

Plasmid

#118320

Purpose

Expression of human Rap2b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap2b (RAP2B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-HisB-GGvT

Plasmid

#87362

Purpose

Expresses GGvT, a tandem homodimer green fluorescent protein

Depositor

Robert Campbell

Article

Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

Insert

GGvT

Tags

His-Tag

Expression

Bacterial

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCDF_HA_CASP_sigma

Plasmid

#197614

Purpose

For expression of HA-tagged CASP sigma in E. coli

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

HA-tagged CASP sigma

Expression

Bacterial

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+Flag-XTCF3-HA

Plasmid

#32998

Depositor

Sergei Sokol

Article

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

XTCF3 (tcf3 Frog)

Tags

Flag and HA

Expression

Mammalian

Promoter

sCMV IE94

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pmCherry-U6-CLIC3

Plasmid

#140583

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CLIC3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMAH-Golgi-bapaUGAc

Plasmid

#201675

Purpose

boronic acid- and peptide-assisted UDP-GlcNAc sensor in Golgi

Depositor

Huiwang Ai

Article

Zhang et al ACS Sens. 2023 Jul 22. doi: 10.1021/acss

Insert

Golgi-bapaUGAc

Tags

Golgi leading sequence

Expression

Mammalian

Promoter

CMV SP6

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pNUT1

Plasmid

#175746

Purpose

Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 1

Depositor

Yubin Zhou

Article

Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

Insert

mCherry-LOV2-NoLS (HIV Rev transactivating protein)

Tags

mCherry

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMOLUC

Plasmid

#12514

Depositor

Tong-Chuan He

Article

Feng et al Biotechniques. 2002 May . 32(5):992, 994

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only

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