We narrowed to 10,414 results for: 158

1. pKH143_EGFP-YIPF3 LIR mut

   Plasmid

   #214975

   Purpose

   GFP tagged LIR mutant YIPF3

   Depositor

   Wade Harper

   Article

   Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

   Insert

   GFP-YIPF3_mut (YIPF3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   LIR mutant

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-puro-PARG shRNA

   Plasmid

   #23260

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   poly(ADP-ribose) glycohydrolase shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

3. ShadowG-SUMO1

   Plasmid

   #175239

   Purpose

   Bacterial expression and purification, conjugatable SUMO for SUMOylation

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXWJ109HgAmp30E

   Plasmid

   #123149

   Purpose

   Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, PmerT, PhrpLE

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

   Plasmid

   #112921

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUSE-DEST_R1R4

   Plasmid

   #97186

   Purpose

   For fusion gene cloning. Recombine Gene A + Gene B into this Gateway Destination Vector

   Depositor

   Kenneth Scott

   Article

   Lu et al Cancer Res. 2017 May 16. pii: canres.274

   Type

   Empty backbone

   Use

   Lentiviral; Gateway

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. piGECI-NES-N1

   Plasmid

   #160422

   Purpose

   Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

   Plasmid

   #153302

   Purpose

   Expresses ClpP protease in the cell-free TX-TL system

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Insert

   clpP (clpP Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. K401-iLid

   Plasmid

   #122484

   Purpose

   Component of optically-induced motor dimerization system

   Depositor

   Matt Thomson

   Article

   Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

   Insert

   Kinesin 1-401 iLid His tag
   

   Use

   Tags

   His6 and iLid

   Expression

   Bacterial

   Mutation

   Kinesin amino acids 1-401

   Promoter

   T7

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKH163_pSmart-mNEON-YIPF4

    Plasmid

    #214982

    Purpose

    endogenous mneon tag for YIPF4

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    mNEON with YIPF4 homology (YIPF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP-CD28TMD-NTEVp(H75S)

    Plasmid

    #137828

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(H75S)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Mutated Histidine 75 to Serine in NTEVp fragment

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_003 Intergenic control guide 2

    Plasmid

    #193585

    Purpose

    sgRNA control; induces CAS9 cutting in an intergenic region

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgRNA control
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXPR_003 sgNFkB2 guide 2

    Plasmid

    #193594

    Purpose

    NFkB2 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgNFkB2 guide 2 (NFKB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FKBP_cPPVp

    Plasmid

    #118967

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with FKBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRB_nPPVp

    Plasmid

    #118966

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with FRB
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEB2-mNeptune2

    Plasmid

    #104012

    Purpose

    Plasmid encoding mNeptune2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mNeptune2
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (MSKGEE LIKENM… R32…

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IP/Venus-mDFCP1

    Plasmid

    #58742

    Purpose

    Expresses Venus-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pSLS.416

    Plasmid

    #187207

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2)
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7/Lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TaRGET-ABE-C3.1 ver4.0

    Plasmid

    #188250

    Purpose

    Hypercompact adenine base editors

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

    Insert

    TaRGET-ABE3.1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dCas9-NLS-gs10-cGR2176

    Plasmid

    #188258

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cGR2176
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCR1063

    Plasmid

    #111091

    Purpose

    Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    dCas9-KRAB
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-NLS-FRB-VP16

    Plasmid

    #210515

    Purpose

    expresses NLS-FRB-VP16 component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLS-FRB-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PML-ER-pBabe

    Plasmid

    #73556

    Purpose

    vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    ER

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

24. CP1028-ABE8e-dimer

    Plasmid

    #138498

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBR322-Pdp1_NTD-ZFN_L-HiBiT

    Plasmid

    #198319

    Purpose

    Loads HiBiT-tagged L fragment of a zinc finger nuclease specific for human AAVS1 locus using Pdp1 packaging domain

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-ZFN_L-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHR- flag-Lag16-DLL1

    Plasmid

    #205201

    Purpose

    This vector encodes of the synCAM (DLL-1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM DLL1 and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Lenti-evoA1-seBEC

    Plasmid

    #174702

    Purpose

    Small-molecule controlled base editing

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nat Chem Biol. 2021 Dec;17(12):1262-1270

    Insert

    Lenti-evoA1-seBEC
    

    Use

    Lentiviral

    Tags

    flag tag

    Expression

    Mutation

    UGI-E11K

    Promoter

    EFS

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CDK4mut(K35M)-3XFlag-pBabe

    Plasmid

    #73559

    Purpose

    vector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK4mut(K35M)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRX-IP/SECFP-mDFCP1

    Plasmid

    #58765

    Purpose

    Expresses SECFP-mDFCP1 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    ZFYVE1 zinc finger, FYVE domain containing 1 (Zfyve1 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pKD280

    Plasmid

    #125071

    Purpose

    Expression of SO_4387 under Ptac

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    SO_4387 (SO_4387 )
    lacI (lacI )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    S286L- Please see depositor comments below

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge