We narrowed to 10,236 results for: ada

1. pCAG-mTcte1-PA

   Plasmid

   #176469

   Purpose

   Expression vector of mouse t-complex-associated testis expressed 1 (Tcte1) tagged with PA at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   t-complex-associated testis expressed 1 (Tcte1 Mouse)
   

   Tags

   PA

   Expression

   Mammalian

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT/TO-RNF185-GFP

   Plasmid

   #113498

   Purpose

   Expression of human RNF185 with C-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   RNF185 (RNF185 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. iRFP713-N2-Frankenbody-Flag

   Plasmid

   #175291

   Purpose

   Visualize endogeneous loci coupled with ZF probes harboring 10xFLAG tag

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with iRFP713
   

   Tags

   iRFP713

   Expression

   Mammalian

   Available Since

   Dec. 3, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCAGGS-CIBN-EGFP-KRasCT

   Plasmid

   #178527

   Purpose

   Expresses CIBN-EGFP-KRasCT in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

   Insert

   CIBN-EGFP-KRasCT
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-mEGFP-mTrim21ΔRING-Box

   Plasmid

   #105520

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-Neo-gp78/JM20

   Plasmid

   #13303

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78 (AMFR Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5FRT/TO-SVIP-Strep-HA

   Plasmid

   #113491

   Purpose

   Expression of human SVIP with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   SVIP (SVIP Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N2-RIPK1 I539D

   Plasmid

   #78848

   Purpose

   To detect localization of RIPK1 I539D protein in live mammalian cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   I539D

   Promoter

   CMV

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pHDR_NANOS3-T2A-mVenus-PuroTK

   Plasmid

   #222903

   Purpose

   Homology directed repair template for knocking in mVenus reporter to NANOS3.

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   mVenus
   

   Use

   CRISPR

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGE2.0-i

    Plasmid

    #194639

    Purpose

    Empty pAGE2.0 plasmid with pBAD inducible promoter

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV10.KN

    Plasmid

    #63209

    Purpose

    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. EK0445 SFFV-mCherry-SGc(DNAJB1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191155

    Purpose

    Expression of a sensor for human DNAJB1 (hsp40) 3' UTR in mammalian cells; mCherry marker, EGFP output, three MS2 sequences in 3' UTR

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:DNAJB1.s1:EGFP-3xMS2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. WTTEM1_pSTC0-zeo

    Plasmid

    #202351

    Purpose

    Control WT TEM-1 (wild type TEM-1 beta lactamase) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

    Depositor

    Nicholas Gauthier

    Article

    Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

    Insert

    TEM-1
    

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMDJ23 - pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]

    Plasmid

    #159815

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Psmu-2 | eFlp recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas4_1_2_VA

    Plasmid

    #186438

    Purpose

    Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_Anc689-nCas9

    Plasmid

    #163526

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCX-SponGee-Kras

    Plasmid

    #134777

    Purpose

    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    SponGee-Kras
    

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA SF-HERC2 F3

    Plasmid

    #55786

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
    

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only