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pBa.NgCam.GFP

Plasmid

#45061

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

NgCam (L1CAM Chicken)

Tags

eGFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-LacZ

Plasmid

#18816

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-LacZ

Expression

Mammalian

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

Plasmid

#176851

Purpose

lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hYAP1-5SA (YAP1 Human)

Use

Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A (phosphorylation…

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV mCherry-GFP-LC3B G120A

Plasmid

#123235

Purpose

Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

EGFP and mCherry

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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cyto-YFP-FKBPx5

Plasmid

#103777

Purpose

expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-YFP-FKBPx5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTYF-1xGfaABC1D-EGFP

Plasmid

#19974

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

GfaABC1D-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3A S315A

Plasmid

#8350

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

S315A

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-1x

Plasmid

#172281

Purpose

Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-1x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Bcl11a

Plasmid

#139809

Purpose

doxycycline-inducible expression of human Bcl11a in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

BCL11a (BCL11A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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EasyFusion mAID-mCherry with Neo

Plasmid

#113831

Purpose

EasyFusion vector for targeting endogeneous genes with mAID-mCherry

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mAID-mCherry

Use

Synthetic Biology

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_hygro-tagBFP-lox5171

Plasmid

#162070

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Promoter

ADH1/yeast

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-EF1A>Tet3G:IRES:Neo

Plasmid

#198756

Purpose

Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Tet3G, IRES, Neo

Use

Lentiviral

Promoter

EF1A

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-HIFR

Plasmid

#192946

Purpose

Lentiviral production of HIF-1alpha reporter

Depositor

Markus Covert

Article

Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

Insert

HIF-1alpha reporter

Use

Lentiviral

Promoter

mCMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-TRE-CMV-Cre-rtTA

Plasmid

#165457

Purpose

Tetracycline or doxycycline inducible CRE expression from AAVS1 locus

Depositor

Madeline Lancaster

Article

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

CRE

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Promoter

MSCV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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ORF3a-EGFP

Plasmid

#165121

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

ORF3a (ORF3a codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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