We narrowed to 6,019 results for: RIK

1. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

   Plasmid

   #127865

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Mutation

   deleted the c-terminal 20 amino acids

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD mApple

   Plasmid

   #91772

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   monomeric Apple
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   R97K, E152S, A180S, V207T compared to mApple (FP …

   Promoter

   araBAD

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-Cln6

   Plasmid

   #78109

   Purpose

   mammalian expression of mouse Cln6 with C terminal EGFP fusion

   Depositor

   Thomas Braulke

   Article

   Thelen et al PLoS One. 2012;7(4):e35493. doi: 10.1371

   Insert

   Cln6 (Cln6 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

   Plasmid

   #105037

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nprl2 (Nprl2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

   Plasmid

   #105038

   Purpose

   Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Nprl2 (Nprl2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mouse GFP-PRMT7 Wild Type

   Plasmid

   #65056

   Purpose

   Expresses wild type mouse GFP-PRMT7

   Depositor

   Steven Clarke

   Article

   Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

   Insert

   PRMT7 (Prmt7 Mouse)
   

   Use

   Bacterial propagation

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. mTFP1-H1-10

   Plasmid

   #55487

   Purpose

   Localization: Nucleus/Histones, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Insert

   H1 (H1f0 Mouse)
   

   Tags

   mTFP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

   Plasmid

   #127866

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Mutation

   C-terminal 20 amino acids only

   Promoter

   hSyn

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sema7a-AP-His

   Plasmid

   #72049

   Purpose

   Expresses the extracellular region of the Sema7A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema7a (Sema7a Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRetroX-TRE3G_NT-GSDMD_I105N_

    Plasmid

    #201174

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Mutation

    aa1-276 only, I105N

    Promoter

    TRE3G

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-Prom1 D-5

    Plasmid

    #184017

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 641 to 655.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-5 (Prom1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAdTrack: shRev-Erbalpha

    Plasmid

    #22749

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Adenoviral and RNAi

    Expression

    Mammalian

    Available Since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCEP4-myc-HLA-A2-VN

    Plasmid

    #135485

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*02:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T-1 3xHA-MKK7a1-EE

    Plasmid

    #47578

    Purpose

    Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

    Depositor

    Kevin Janes

    Article

    Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

    Insert

    MKK7a1-EE (Map2k7 Mouse)
    

    Tags

    3xHA and GST

    Expression

    Bacterial

    Mutation

    Residues 73-420 of RefSeq sequence. Ser271 to Gl…

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-DYRK2-2A-GFP

    Plasmid

    #118284

    Purpose

    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and GFP

    Expression

    Mammalian

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF3454

    Plasmid

    #144930

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF653 (ZNF653 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-GSDMD_NoCys-Myc

    Plasmid

    #201169

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Mutation

    C39A, C57A, C77A, C122A, C265A, C192A

    Promoter

    MESV

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa.GFP-KIF17 tail 400-1038

    Plasmid

    #134617

    Purpose

    Mammalian expression of mouse KIF1A 400-1038

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF17 (Kif17 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sema6a.a-Fc-His

    Plasmid

    #72163

    Purpose

    Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6a.a (Sema6a Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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