We narrowed to 13,816 results for: cdna

Showing: 4761 - 4780 of 13816 results

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pcDNA3-mCherry-Rheb(C181S)

Plasmid

#192437

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Y951F-VEGFR2-mCherry

Plasmid

#139607

Purpose

Expresses a mutant (Y951F) VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HER3-DMVF-181-R

Plasmid

#87621

Purpose

C-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.

Depositor

Jin Zhang

Article

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

HER3-DMVF181-C (ERBB3 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MTS-KD-c-Src-FLAG

Plasmid

#44653

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (MTS…

Expression

Mammalian

Mutation

changed lysine 298 to methionine

Promoter

CMV promoter

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa120-433

Plasmid

#73411

Purpose

Expresses HA tagged aa120-433 AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 120-433 of AKT1

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-eEF2K(S441A/S445A)

Plasmid

#110161

Purpose

Expression of human eEF2K (S441A/S445A) in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Tags

HA

Expression

Mammalian

Mutation

S441A/S445A

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (250-536) HA

Plasmid

#42209

Depositor

Robert Lefkowitz

Article

Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

Insert

c-SRC (250-536) HA (SRC Human)

Tags

HA

Expression

Mammalian

Mutation

contains amino acids 250-536

Promoter

CMV

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-myc- GFP-Flag

Plasmid

#82471

Purpose

Expresses myc-GFP-Flag

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Myc-GFP-Flag

Tags

Flag and Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

Plasmid

#115789

Purpose

Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

NDP (NDP Human)

Tags

alkaline phosphatase and flag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LAP2-YFP-NLS

Plasmid

#25847

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LAP2-YFP-NLS

Tags

His and NLS

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoL-CaM

Plasmid

#193830

Purpose

CMV overexpression of ChemoL-CaM in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-CaM

Expression

Mammalian

Mutation

EGFP:A206K, E299D; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRIPK1-Flag-delN

Plasmid

#112492

Purpose

Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

Depositor

Junying Yuan

Insert

RIPK1 (RIPK1 Human)

Tags

Flag

Expression

Mammalian

Mutation

deletion of N terminus (AA1-274 deleted)

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-mRIPK1-S321E

Plasmid

#115340

Purpose

Mammalian expression of Flag-mRIPK1-S321E

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S321E

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nprot-HF_GC3opt

Plasmid

#157732

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nprot-HF_GC3opt (N SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.2/V5 hsa-mir-124

Plasmid

#26306

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

hsa-mir-124 (MIR124-1 Human)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HCMVgH-8his (Merlin)

Plasmid

#136447

Purpose

Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein H (UL75 Human betaherpesvirus 5)

Tags

Short GGSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular domain (a.a. 1-716). Codon-optimize…

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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GLUT9-eGFP/pcDNA-DEST47

Plasmid

#18730

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 AURKA Lys162Met

Plasmid

#157751

Purpose

Expression of kinase-dead AURKA K62M in mammalian cells

Depositor

Marc Tramier

Article

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Insert

AURKA (AURKA Human)

Expression

Mammalian

Mutation

AURKA K162M is a kinase-dead version of AURKA

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Expression

Mammalian

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3A

Plasmid

#50854

Purpose

expresses constituvely active cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3A (CFL1 Human)

Expression

Mammalian

Mutation

constitutively active, S3A

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits