We narrowed to 4,396 results for: 256
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Plasmid#202566PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal mNeonGreen2 fluorophore on the ORF1 protein for fluorescence microscopyDepositorInsertLINE-1
TagsmNeonGreen2 on ORF1pExpressionMammalianAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-UCHL1
Plasmid#22563DepositorAvailable SinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Camui-CR
Plasmid#40256DepositorInsertCamuiα-CR
ExpressionMammalianPromoterCMV IEAvailable SinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-dCas9
Plasmid#125687PurposeGene knockdown for actinomycetesDepositorInsertstreptomyces codon optimized spdCas9, sgRNA casette
UseCRISPR and Synthetic BiologyPromoterermE*/tipAAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-ChR2-EYFP-Kv
Plasmid#89256PurposeChannelrhodopsin localized to neuronal soma via a Kv2.1 targeting motifDepositorInsertChannelrhodopsin
UseAAVTagsEYFPExpressionMammalianMutationH134RPromotersynapsinAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromoterU6Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP11
Plasmid#22566DepositorAvailable SinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_HelperΔgIII
Plasmid#190433PurposeM13 helper genes cloned into the pSEVA631 vector, except the minor coat protein coding gene gIII (also called gp3, or g3p). This plasmid does not carry an M13 packaging signal.DepositorInsertM13 genes I-V and VII-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63_Helper
Plasmid#190432PurposeM13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ297.)DepositorInsertM13 genes: I-XI
UseSynthetic BiologyAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT374
Plasmid#125615PurposeExpresses evoFERNY-BE4max-NG in mammalian cellsDepositorInsertevoFERNY-BE4max-NG
ExpressionMammalianMutationH102P D104NAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJE-Ma pylRS nitroY/haloY-F5
Plasmid#225684PurposeExpresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control during Ma Pyl tRNA-synthetase validation.DepositorInsertsM. alvus PylRS 3-NY/HaloY F5
M. alvus Pyl-tRNA(6)
UseMethanomethylophilus alvusMutationCTG125CTT, N166A, V168S, A223C, W239RPromoterGlnS and LppAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRL278
Plasmid#70256PurposesacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalAvailable SinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTK229
Plasmid#71256Purposeluciferase reposter with 229 bp of the HSV TK promoterDepositorInsertThymidine Kinase promoter
UseLuciferaseTagsluciferaseExpressionMammalianPromoterHSV Thymidine KinaseAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT376
Plasmid#125617PurposeExpresses BE4max-NG in mammalian cellsDepositorInsertBE4max-NG
ExpressionMammalianAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
NRASA
Plasmid#25256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNRASA:M1-N172 (NRAS Human)
TagsHisExpressionBacterialMutationContains amino acids M1-N172Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF HaloTag-SYT1
Plasmid#197280PurposeEncodes full-length wild type synaptotagmin 1 (SYT1) with an N-terminal HaloTagDepositorAvailable SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFsynW KDEL Hook
Plasmid#197277PurposeEncodes ER-targeted streptavidin-KDEL hook (RUSH system).DepositorInsertStreptavidin
UseLentiviralTagsFLAGPromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B7-deltaE4
Plasmid#122560PurposeModified block 7, with deletion in E4DepositorInsertAdenovirus 5 genomic region 30888-35938
UseSynthetic BiologyMutationAdenovirus sequences 32874-35461 replaced with Sp…Available SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFsynW TeTx-LC
Plasmid#197284PurposeEncodes for viral expression of the light chain of tetanus toxin.DepositorInsertTetanus toxin light chain
UseLentiviralMutationN/APromoterhSyn (human synapsin I promoter)Available SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mEGFP
Plasmid#132565PurposemEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)DepositorInsertmEGFP
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only