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pFF749

Plasmid

#61452

Purpose

IPTG-inducible msd_kanR(ON)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)_dRT

Use

Synthetic Biology

Mutation

D197A D198A

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pSL6 - Brr2 del247 WT

Plasmid

#90256

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pFF761

Plasmid

#61455

Purpose

aTc-inducible SCRIBE_galK(OFF)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(OFF)

Use

Synthetic Biology

Promoter

pTetO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICH41258_EYFP

Plasmid

#232567

Purpose

Level-0 module; EYFP

Depositor

Andreas Houben

Article

Somasundaram et al Plant Communications, 30 Mar 2026: 10183

Insert

EYFP

Use

Coding sequence

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAGM1299_EL5

Plasmid

#232569

Purpose

Level-0 module; EL5

Depositor

Andreas Houben

Article

Somasundaram et al Plant Communications, 30 Mar 2026: 10183

Insert

EL5

Use

Coding sequence

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-RAD51

Plasmid

#222567

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of RAD51

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

RAD51 (RAD51 Human)

Expression

Mammalian

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-iRFP713-HO1

Plasmid

#197256

Purpose

Expresses the protein of iRFP713 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

iRFP713

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pTorD-H6

Plasmid

#169039

Purpose

Escherichia coli TorD with C-terminal 6xHis in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

TorD-H6

Tags

6xHis

Expression

Bacterial

Mutation

The mutation N at position 46 was changed back to…

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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A0A090SY56

Plasmid

#163256

Purpose

Inducible expression of UniProt identifier A0A090SY56

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A0A090SY56

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMXBK_aFb

Plasmid

#114256

Purpose

POC12398, MetClo assembly vector with F replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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TAL3082

Plasmid

#41256

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-gbas-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pRAJ31m

Plasmid

#39256

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ31 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pFsynW KDEL Hook

Plasmid

#197277

Purpose

Encodes ER-targeted streptavidin-KDEL hook (RUSH system).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Streptavidin

Use

Lentiviral

Tags

FLAG

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-UCHL1

Plasmid

#22563

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

UCHL1 (UCHL1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP11

Plasmid

#22566

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEF SYT1-HaloTag

Plasmid

#197281

Purpose

Encodes full-length wild type synaptotagmin 1 (SYT1) with a C-terminal HaloTag

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Expression

Mammalian

Mutation

N/A

Promoter

EF1alpha

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBT374

Plasmid

#125615

Purpose

Expresses evoFERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max-NG

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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PB-sgRNA(MS2)

Plasmid

#102560

Purpose

Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Ying Liu

Article

Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

Type

Empty backbone

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Camui-CR

Plasmid

#40256

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Camuiα-CR

Expression

Mammalian

Promoter

CMV IE

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only

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